Diagnostic value of human papillomavirus E6/E7 mRNA for residue and recurrence after cervical conization.
10.11817/j.issn.1672-7347.2016.06.009
- Author:
Hua ZHAO
1
;
Huajiang SHAO
2
Author Information
1. Medical School of Ningbo University, Ningbo Zhejiang 315000, China.
2. Department of Obstetrics & Gynecology, Yangming Hospital Affiliated to Medical School of Ningbo University, Yuyao Zhejiang 315400, China.
- Publication Type:Journal Article
- MeSH:
Cervical Intraepithelial Neoplasia;
Conization;
Female;
Humans;
Neoplasm Recurrence, Local;
Papillomaviridae;
Papillomavirus Infections;
RNA, Messenger;
RNA, Viral;
Uterine Cervical Neoplasms
- From:
Journal of Central South University(Medical Sciences)
2016;41(6):606-611
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the diagnostic value of human papillomavirus E6/E7 mRNA for residue and recurrence of cervical intraepithelial neoplasia (CIN) patients after cervical conization.
METHODS:A total of 154 patients, who underwent cervical conization and possessed complete follow-up data of high-grade cervical intraepithelial neoplasia (CINII, III), were subjected to thin-prep cytology test (TCT), HPV-DNA typing and HPV E6/E7 mRNA test in 3-6 months and 12 months after cervical conization. Abnormal cytology (≥ASC-US) or high-risk HPV-DNA (+) or HPV E6/E7 mRNA (+) cases were found by colposcopy and cervical biopsy pathological diagnosis.
RESULTS:Nine patients had residue and 22 recurred. HPV-DNA detections after cervical conization in 57 patients were positive. Among them, 30 patients had residual/recurrent lesions. HPV E6/E7 mRNA detections in 26 patients were positive. Among them, 24 patients had residual/recurrent lesions. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of diagnosis in the detection of HPV-DNA were 96.8%, 78%, 52.6%, 99%, and 81.8%, respectively. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of diagnosis in the detection of HPV E6/E7 mRNA were 77.4%, 98.4%, 92.3%, 94.5%, and 94.2%, respectively. The specificity and positive predictive value of HPV E6/E7 mRNA test were greater than those of HPV-DNA typing test. There was a significant difference between the two groups (P<0.05).
CONCLUSION:Detection of HPV E6/E7 mRNA during the follow-up period could timely and effectively forecast the risk of residue and recurrence of CIN after cervical conization, and reduce excessive examination and treatment.
