Effect of H. pylori on the expression of CCAAT enhancer binding protein α and Cx43 and its role
in gastric carcinogenesis.
10.11817/j.issn.1672-7347.2016.07.007
- Author:
Li ZHOU
1
,
2
;
Canxia XU
1
,
3
;
Tingzi HU
4
;
Xiaoming LIU
4
;
Jing XIAO
4
;
Ling LUO
4
;
Xiaoxia JIANG
4
Author Information
1. Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha 410013
2. Department of Internal Medicine, Hunan Provincial Maternal and Child Health Care Hospital, Changsha 410008,China.
3. Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha 410013, China.
4. Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
CCAAT-Enhancer-Binding Protein-alpha;
Cell Line;
Cell Transformation, Neoplastic;
Connexin 43;
Down-Regulation;
Gastric Mucosa;
Helicobacter Infections;
Helicobacter pylori;
Humans;
RNA, Messenger;
Real-Time Polymerase Chain Reaction;
Stomach Neoplasms
- From:
Journal of Central South University(Medical Sciences)
2016;41(7):700-706
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of H. pylori on the expression of CCAAT enhancer binding protein α (C/EBPα) and connexin 43 (Cx43) in gastric carcinogenesis.
METHODS:Different gastric mucosal epithelial cell lines (GES-1 cells, AGS cells and SGC-7901 cells) were cultured. A total of 6 cases of chronic non-atrophic gastritis tissues, 12 cases of gastric precancerous lesions tissues and 12 cases of gastric cancer tissues were collected under endoscopy. The expression of C/EBPα and Cx43 mRNA in the above-mentioned cells and tissues was detected by real-time PCR. The GES-1 cells and East Asian CagA+ H. pylori strains were co-cultured for 24 and 48 h as an experimental group, and those cell lines without H. pylori infection were cultured for 24 and 48 h as a control group. Real-time PCR and Western bolt were used to detect the expression of mRNA and proteins of C/EBPα and Cx43 in GES-1 cells, respectively.
RESULTS:The expressions of C/EBPα and Cx43 mRNA in the AGS and SGC-7901 cells were lower than those in GES-1 cells (all P<0.05), and both of them decreased more profoundly in the SGC-7901 cells than those in the AGS cells (both P<0.05). The expressions of C/EBPα and Cx43 mRNA were lower in the gastric cancer tissues than those in the chronic non-atrophic gastritis tissues (both P<0.05) and gastric precancerous lesion tissues (both P<0.05). The C/EBPα expression was positively correlated with Cx43 expression (gastric precancerous lesion tissues: r=0.679, gastric cancer tissues: r=0.792; both P<0.05). Compared with the control group, the expressions of C/EBPα and Cx43 mRNA and protein in the experimental group were decreased at 24 and 48 h after culture (both P<0.05), which were lower at 48 h than those at 24 h (both P<0.05).
CONCLUSION:H. pylori infection can down-regulate the expressions of C/EBPα and Cx43 on gastric epithelial cells, which may be associated with gastric carcinogenesis.
