miR-181a promotes the proliferation and metastasis of osteosarcoma cells by targeting RASSF1A.
10.11817/j.issn.1672-7347.2016.08.003
- Author:
Xiaofang ZANG
1
;
Qin LI
1
;
Weiguo WANG
1
;
Yong ZHOU
1
;
Shijie CHEN
1
;
Tao XIAO
2
Author Information
1. Department of Orthopaedics, Third Xiangya Hospital, Central South University, Changsha 410013, China.
2. Department of Orthopaedics, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Cell Proliferation;
Down-Regulation;
Humans;
MicroRNAs;
Neoplasm Metastasis;
Osteosarcoma;
Real-Time Polymerase Chain Reaction;
Transfection;
Tumor Suppressor Proteins;
Up-Regulation
- From:
Journal of Central South University(Medical Sciences)
2016;41(8):789-795
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the role of miR-181a in promoting the proliferation and metastasis of osteosarcoma cells by targeting RASSF1A.
METHODS:The level of miR-181a in 30 human osteosarcoma tissues and corresponding bone tissues was detected by real-time PCR, and the correlation between the level of miR-181a and clinicopathological characteristics of osteosarcoma was analyzed. Osteosarcoma cells MG-63 were transfected with chemically-synthesized miR-181a mimics and inhibitors, and the proliferation, migration and invasion of MG-63 cells were detected by MTT and Transwell assay. The specific binding ability of miR-181a to RASSF1A 3'-UTR was theoretically predicted and detected by the dual luciferase reporter gene assay.
RESULTS:The level of miR-181a in osteosarcoma tissues was statistically higher than that in the corresponding bone tissues (P<0.001). However, the level of miR-181a was not correlated with gender, age, tumor size and stage (P>0.05). MTT and Transwell assays showed that the growth rate, migration and invasion ability of MG-63 cells with up-regulation of miR-181a was significantly increased compared with negative control (P<0.05), while the growth rate, migration and invasion ability of MG-63 with down-regulated miR-181a was significantly decreased compared with negative control (P<0.05). Luciferase reporter gene assay showed that miR-181a targeted the 3'-UTR of RASSF1A and regulated the expression of RASSF1A.
CONCLUSION:MiR-181a promotes the proliferation and metastasis of osteosarcoma cells through specifically binding to RASSF1A 3'-UTR and subsequent down-regulation of RASSF1A.
