Feasibility analysis of quantitative detection on serum HBeAg/HBeAb by time-resolved immunofluorescence assay.
10.11817/j.issn.1672-7347.2016.08.013
- Author:
Xiaoyu FU
1
,
2
;
Feiyuan WU
3
;
Gang CHEN
3
;
Yanling XIE
3
;
Guohua DENG
3
;
Shaojun GAN
3
;
Lei FU
1
,
2
Author Information
1. Department of Infectious Disease, Xiangya Hospital
2. Hunan Key Laboratory of Viral Hepatitis, Central South University, Changsha 410008, China.
3. Third Department of Internal Disease, Ganxi Hospital, Pingxiang Jiangxi 337019, China.
- Publication Type:Journal Article
- MeSH:
Feasibility Studies;
Fluorescent Antibody Technique;
Hepatitis B;
Hepatitis B Antibodies;
Hepatitis B e Antigens;
Hepatitis B virus;
Humans
- From:
Journal of Central South University(Medical Sciences)
2016;41(8):852-855
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine whether time-resolved immunofluorescence assay (TRIFA) shares the similar accuracy and specificity with automatic chemiluminescence immunoassay (CMIA) in analyzing HBeAg levels in hepatitis B.
METHODS:A total of 157 serum samples were collected from outpatients with infection of HBV in Xiangya Hospital, Central South University. CMIA and TRIFA were used to analyze HBeAg quantitation and HBeAg/HBeAb qualitative detection, respectively.
RESULTS:The linear regression equation for the two methods was Y=0.72779X-4.0551 (r=0.712, P<0.001). Compared with the CMIA, the sensitivity and specificity in detection of HBeAg by TRIFA were 89.89% and 100%, respectively, and the coincidence rate of HBeAg was 94.27% by two assays. Similarly, the sensitivity and specificity in detection of HBeAb by TRIFA were 100% and 95.45%, respectively. The coincidence rate was 97.45% by two assays.
CONCLUSION:TRIFA has similar accuracy, sensitivity, and specificity with CMIA in quantitative detection of HBeAg, and their coincidence rate in detection of HBeAg/HBeAb is high.
