Feasibility analysis of quantitative detection on serum HBeAg/HBeAb by time-resolved immunofluorescence assay.
10.11817/j.issn.1672-7347.2016.08.013
- Author:
Xiaoyu FU
1
;
Feiyuan WU
2
;
Gang CHEN
2
;
Yanling XIE
2
;
Guohua DENG
2
;
Shaojun GAN
2
;
Lei FU
1
Author Information
1. Department of Infectious Disease, Xiangya Hospital; Hunan Key Laboratory of Viral Hepatitis, Central South University, Changsha 410008, China.
2. Third Department of Internal Disease, Ganxi Hospital, Pingxiang Jiangxi 337019, China.
- Publication Type:Journal Article
- MeSH:
Feasibility Studies;
Fluorescent Antibody Technique;
Hepatitis B;
Hepatitis B Antibodies;
Hepatitis B e Antigens;
Hepatitis B virus;
Humans
- From:
Journal of Central South University(Medical Sciences)
2016;41(8):852-855
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine whether time-resolved immunofluorescence assay (TRIFA) shares the similar accuracy and specificity with automatic chemiluminescence immunoassay (CMIA) in analyzing HBeAg levels in hepatitis B.
METHODS:A total of 157 serum samples were collected from outpatients with infection of HBV in Xiangya Hospital, Central South University. CMIA and TRIFA were used to analyze HBeAg quantitation and HBeAg/HBeAb qualitative detection, respectively.
RESULTS:The linear regression equation for the two methods was Y=0.72779X-4.0551 (r=0.712, P<0.001). Compared with the CMIA, the sensitivity and specificity in detection of HBeAg by TRIFA were 89.89% and 100%, respectively, and the coincidence rate of HBeAg was 94.27% by two assays. Similarly, the sensitivity and specificity in detection of HBeAb by TRIFA were 100% and 95.45%, respectively. The coincidence rate was 97.45% by two assays.
CONCLUSION:TRIFA has similar accuracy, sensitivity, and specificity with CMIA in quantitative detection of HBeAg, and their coincidence rate in detection of HBeAg/HBeAb is high.
