Interaction between functional nano-hydroxyapatite and cells and the underlying mechanisms.
10.11817/j.issn.1672-7347.2016.09.008
- Author:
Min YANG
1
;
Yanzhong ZHAO
2
,
3
;
Guohui WANG
1
;
Juan TAN
1
;
Shaihong ZHU
2
,
3
Author Information
1. Medical Experimental Center, Third Xiangya Hospital, Central South University, Changsha 410013, China.
2. Medical Experimental Center, Third Xiangya Hospital
3. Research Center for Medical Material and Instruments, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Arginine;
pharmacology;
Biological Transport, Active;
physiology;
Caveolins;
physiology;
Cells, Cultured;
Clathrin;
physiology;
Durapatite;
pharmacokinetics;
Endocytosis;
physiology;
Human Umbilical Vein Endothelial Cells;
cytology;
Humans;
Nanoparticles;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2016;41(9):937-945
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the interaction between arginine functionalized hydroxyapatite (HAP/Arg) nanoparticles and endothelial cells, and to investigate mechanisms for endocytosis kinetics and endocytosis.
METHODS:Human umbilical vein endothelial cells (HUVECs) were selected as the research model.Cellular uptake of HAP/Arg nanoparticles were observed by laser scanning confocal microscopy.Average fluorescence intensity of cells after ingestion with different concentrations of HAP/Arg nanoparticles were determined by flow cytometer and atomic force microscopy.
RESULTS:The HAP/Arg nanoparticles with doped terbium existed in cytoplasm, and most of them distributed around the nucleus area after cellular uptake by HUVECs. Cellular uptake process of HAP/Arg nanoparticles in HUVECs was in a time and concentration dependent manner. 4 h and 50 mg/L was the best condition for uptake. HAP/Arg nanoparticles were easier to be up-taken into the cells than HAP nanoparticles without arginine functionalized.
CONCLUSION:HAP/Arg nanoparticles are internalized by HUVECs cells through an active transport and energy-dependent endocytosis process, and it is up-taken by cells mainly through caveolin-mediated endocytosis, but the clathrin-dependent endocytic pathway is also involved..
