Mechanism of Novaferon on production of TNF-α by monocytes isolated from normal human peripheral blood.
10.3969/j.issn.1672-7347.2013.01.012
- Author:
Fujun LI
1
;
Mei XUE
;
Fanggen LU
;
Yiyou ZOU
Author Information
1. Department of Digestion, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Humans;
Lipopolysaccharides;
pharmacology;
Monocytes;
drug effects;
metabolism;
NF-kappa B;
genetics;
metabolism;
RNA, Messenger;
genetics;
metabolism;
Recombinant Proteins;
pharmacology;
Tumor Necrosis Factor-alpha;
antagonists & inhibitors;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2013;38(1):66-69
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the role of Novaferon on TNF-α production and expression of NF-κB mRNA in monocytes isolated from normal human peripheral blood and to provide theoretical basis for treatment of immunological diseases with Novaferon.
METHODS:Monocytes were isolated from the peripheral blood in 30 healthy volunteers and divided into 5 groups: group A was blank control, group B was stimulated by LPS without Novaferon intervention, group C by LPS together with Novaferon intervention, group D by LPS before Novaferon intervention, which group E by LPS after Novaferon intervention. We detected the concentration of TNF-α after LPS stimulation and Novaferon intervention in the supernatant by ELISA and expression of NF-κB mRNA by RT-PCR.
RESULTS:Novaferon inhibited TNF-α production by monocytes isolated from healthy volunteers induced by LPS in vitro in group D compared with group B [(1446.76±72.36) pg/mL vs (946.46±46.12) pg/mL, P<0.01], and the rate was 29.7%. There was no significant change in TNF-α concentration in group C and E compared with group B [(1446.76±72.36) pg/mL vs (1275.62±87.75) pg/mL, P>0.05; (1446.76±72.36) pg/mL vs (1383.62±86.96) pg/mL, P>0.05]. There was significant change in NF-κB mRNA expression in group D compared with group B (0.2829±0.0365 vs 0.4994±0.0604, P<0.01). There was no significant change in NF-κB mRNA expression in group C and group E compared with group B (0.4716±0.0616 vs 0.4994±0.0604, P>0.05; 0.4767±0.0600 vs 0.4994±0.0604, P>0.05).
CONCLUSION:Novaferon can suppress TNF-α secretion by monocytes induced by LPS in vitro, and it can affect the immunity function of monocytes, which may be associated with the downregulation of NF-κB mRNA expression in monocytes.
