Effect of DJ-1 siRNA on biological behavior of human lung squamous carcinoma SK-MES-1 cells.
10.3969/j.issn.1672-7347.2013.01.002
- Author:
Wangli WEI
1
;
Can'e TANG
;
Xianquan ZHAN
;
Hong YI
;
Cui LI
Author Information
1. Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Carcinoma, Squamous Cell;
genetics;
pathology;
Cell Line, Tumor;
Cell Movement;
genetics;
Cell Proliferation;
Genetic Vectors;
genetics;
Humans;
Intracellular Signaling Peptides and Proteins;
genetics;
metabolism;
Lentivirus;
genetics;
metabolism;
Lung Neoplasms;
genetics;
pathology;
Molecular Sequence Data;
Oncogene Proteins;
genetics;
metabolism;
Protein Deglycase DJ-1;
RNA Interference;
RNA, Small Interfering;
genetics
- From:
Journal of Central South University(Medical Sciences)
2013;38(1):7-13
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:RNA interference technology (siRNA) was used to inhibit the expression of DJ-1 gene in lung squamous cell carcinoma SK-MES-1 cells, and the cell biological behaviors were investigated to explore the function of DJ-1 gene.
METHODS:A targeted DJ-1 siRNA lentiviral vector with a green fluorescent protein (GFP) as a reporter was constructed. The constructed DJ-1 siRNA and control-siRNA vectors were infected into SK-MES-1 cells as experimental (DJ-1 siRNA) and control (Control siRNA) groups, respectively. The DJ-1 protein expression was determined by Western blot. The cell proliferation capability was measured with methyl thiazolyl tetrazolium (MTT). The cell cycle was analyzed by flow cytometry. The capability of cell migration was determined by Transwell method.
RESULTS:Compared with control-siRNA and blank-control groups, the protein expression of DJ-1 gene was down-regulated, the capability of cell proliferation was obviously inhibited (P<0.01), the cell cycle was arrested with increased number of G1- and G2-phase cells and reduced number of S-phase cells, and the capability of cell migration was significantly decreased (P<0.01) in the DJ-1 siRNA-infected cells.
CONCLUSION:DJ-1 gene might play a role in promoting cell proliferation and cell migration capability in vitro in lung cancer SK-MES-1 cells.
