Construction of special reporter to detect DNA methylation regulatory activity in FCER1G gene promoter through patch-methylation.
10.3969/j.issn.1672-7347.2013.02.002
- Author:
Yunsheng LIANG
1
;
Ming ZHAO
;
Gongping LIANG
;
Heng YIN
;
Qianjin LU
Author Information
1. Department of Dermatology, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
DNA Methylation;
Gene Expression Regulation;
Genes, Reporter;
Humans;
Luciferases;
genetics;
Molecular Sequence Data;
Promoter Regions, Genetic;
genetics;
Receptors, IgE;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2013;38(2):120-124
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To construct a special luciferase reporter to detect DNA methylation regulatory activity in FCER1G gene promoter regulatory element.
METHODS:We constructed special full and mock methylated FCER1G gene promoter regulatory luciferase reporters by patch-methylation, and detected DNA methylation regulatory activity by comparing the luciferase activity of full-methylated luciferase reporters with mock-methylated reporters.
RESULTS:We successfully constructed the full and mock methylated FCER1G gene promoter regulatory luciferase reporters. The ratio of luciferase activity between the full methylated and the mock methylated was (0.36±0.07):1 (P<0.001).
CONCLUSION:FCER1G promoter activity is methylation-sensitive and is regulated by DNA methylation.
