Effect of Naoling decoction on the expression of microglia and IL-6 in hippocampal CA3 region of rats with synthetic Alzheimer's disease.
10.3969/j.issn.1672-7347.2013.02.001
- Author:
Zhe WANG
1
;
Minghui WU
;
Bingwu ZHONG
;
Dongdong ZHANG
;
Mingda HE
Author Information
1. Department of Traditional Chinese Medicine, Central South University, Changsha, China. ericwangzhe@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Alzheimer Disease;
drug therapy;
metabolism;
Animals;
CA3 Region, Hippocampal;
metabolism;
pathology;
Disease Models, Animal;
Drugs, Chinese Herbal;
therapeutic use;
Female;
Interleukin-6;
genetics;
metabolism;
Learning;
drug effects;
Male;
Memory;
drug effects;
Microglia;
pathology;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Central South University(Medical Sciences)
2013;38(2):113-119
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To observe the effect of Naoling decoction on the learning and memory behaviors and the expression of microglia and IL-6 in hippocampal CA3 region of rats with Alzheimer's disease (AD), and to elucidate the potential mechanism.
METHODS:Thirty SD rats were randomly divided into 5 groups: a normal group, a sham-operation group, an AD group, a Naoling decoction group and a Naofukang group. The spatial learning and memory behaviors of the rats were investigated by water maze and Y-maze. The Alzheimer's disease model was established by injecting Aβ1-42 into the hippocamal of the rats. Expression of OX-42 (one of the microglia specific markers) and IL-6 in the CA3 region of hippocamal was measured by immunohistochemical stain.
RESULTS:Morris water maze experiment showed that the escape latency of hidden platform in the AD group was significantly delayed (P<0.05) and the average times of passing was decreased (P<0.05). Y-maze test showed that the times needed to the learn how to evade the electrical stimulation in the AD group was most than in other groups (P<0.05). Compared with the AD group, the Morris water maze test and Y-maze test of the Naoling decoction group were significantly different (P<0.05). The expression of OX-42 and IL-6 in the CA3 region of hippocamal in the Naoling decoction group was decreased (P<0.05).
CONCLUSION:Naoling decoction can improve learning and memory, and weaken the expression of OX-42 and IL-6 in hippocampal CA3 of AD rats, which may partly be the therapeutic mechanism of Naoling decoction for AD.
