Effect of corticotrophin-releasing hormone on connexin-43 phosphorylation and gap junction intercellular communication in human myometrial smooth muscle cells.
10.3969/j.issn.1672-7347.2013.02.008
- Author:
Yun HU
1
;
Xin HE
;
Yiling DING
Author Information
1. Department of Obstetrics and Gynecology, Second Xiangya Hospital, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Cell Communication;
drug effects;
Cells, Cultured;
Connexin 43;
metabolism;
Corticotropin-Releasing Hormone;
pharmacology;
Female;
Gap Junctions;
drug effects;
Humans;
Myocytes, Smooth Muscle;
metabolism;
Myometrium;
cytology;
Phosphorylation;
drug effects
- From:
Journal of Central South University(Medical Sciences)
2013;38(2):155-161
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effect of human corticotrophin-releasing hormone (CRH) on the expression of connexin-43 phosphate (P-Cx43) in human myometrial smooth muscle cells (SMCs) and the function of cell gap junction intercellular communication in SMCs.
METHODS:Human non-conceive myometial SMCs were cultured with different concentrations of CRH (0, 5.85, 58.5, 585 and 5850 pmol/L). Western blot was used to test P-Cx43 and Cx43 non-phosphate (NP-Cx43) of protein expression. Cell scratch was used to test cell gap junction intercellular communication opening status in human myometrial SMCs.
RESULTS:Compared with the control group, the expression of P-Cx43 was higher in the CRH groups (P<0.01), and was concentration-dependent. There was no significant difference in NPCx43 between the control group and the CRH groups (P>0.05). The transmission of cell layers in the CRH groups was higher than that in the control group (P<0.01), and as the concentration of CRH increased, the time was concentration-dependent (P<0.01).
CONCLUSION:CRH can enhance the expression of P-Cx43 and the function of gap junction intercellular communication in the primary cultured myometrial SMCs.
