Role of hypoxia in viability and endothelial differentiation potential of UC-MSCs and VEGF interference.
10.3969/j.issn.1672-7347.2013.04.001
- Author:
Peng LI
1
;
Changyong ZHOU
;
Lei YIN
;
Xianqin MENG
;
Lina ZHANG
Author Information
1. Department of Emergency Room, Qingdao University, Qingdao Shandong, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Differentiation;
drug effects;
Cell Hypoxia;
Cell Proliferation;
Cell Survival;
Cells, Cultured;
Humans;
Mesenchymal Stem Cells;
cytology;
Protective Agents;
pharmacology;
Reactive Oxygen Species;
metabolism;
Umbilical Cord;
cytology;
Vascular Endothelial Growth Factor A;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2013;38(4):329-340
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the effect of hypoxia on cell viability and the endothelial differentiation potential in human umbilical cord derived mesenchymal stem cells (UC-MSCs), and to assess the in vitro protective role of VEGF under low oxygen tension.
METHODS:MSCs were isolated from human umbilical cords and cultured in vitro. The morphological and phenotypic characterizations of human UC-MSCs were analyzed. The hypoxia induction was performed with or without the presence of 50 ng/mL of VEGF for different lengths of time. The cell proliferation, apoptosis, and reactive oxygen species (ROS) generation were assessed. Meanwhile, the endothelial differentiation potential of the UC-MSCs was measured.
RESULTS:An increased apoptosis and ROS generation but reduced proliferation rate were observed at early stages (6, 12 h) after transferring the UC-MSCs from the atmospheric condition to the hypoxia condition. However, the UC-MSCs presented equal proliferation and apoptosis levels under hypoxic condition as compared with those under the atmospheric condition at the later stages (24, 72 h). A high concentration of exogenous VEGF (50 ng/mL) attenuated the increased apoptosis and inhibited the proliferation of UC-MSCs, induced by a short-term hypoxia treatment. After 14 days of exogenous VEGF induction under the hypoxia condition, the UC-MSCs acquired an early endothelial phenotype consisting of a mature endothelial molecule and some endothelial functions.
CONCLUSION:UC-MSCs progressively adapt to hypoxia in a step-by-step manner and maintain differentiation potential under hypoxia condition. VEGF can protect the UC-MSCs from cell damage and induce a differentiation of UC-MSCs toward endothelial lineage under hypoxic conditions.
