Preliminary screen of metastasis-related genes in cervical squamaous cell carcinoma.
10.3969/j.issn.1672-7347.2013.04.012
- Author:
Yilin WU
1
;
Lang ZOU
;
Yi HU
;
Hui DING
Author Information
1. Department of Obstetrics and Gynecology, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Carcinoma, Squamous Cell;
genetics;
pathology;
Female;
Gene Expression Profiling;
Gene Expression Regulation, Neoplastic;
Humans;
Lymphatic Metastasis;
Middle Aged;
Oligonucleotide Array Sequence Analysis;
Uterine Cervical Neoplasms;
genetics;
pathology
- From:
Journal of Central South University(Medical Sciences)
2013;38(4):405-412
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the differentially expressed gene profile in cervical squamous cell carcinoma tissues with and without pelvic lymph node metastasis by microarray technique and to screen tumor metastasis-related genes.
METHODS:Cancer tissue samples were collected from 4 cervical squamous cell carcinoma patients with pelvic lymph metastasis and 6 patients without metastasis before the treatment. Differentially expressed gene profile was identified between cervical squamous cell carcinoma tissues with and without pelvic lymph node metastasis by NimbleGen microarray. Fluorescent real-time quantitative PCR was employed to validate the accuracy of the microarray by detecting the expression of genes picked from the differentially expressed gene profile. We screened tumor metastasis-related genes through the gene bank, PUBMED and gene ontology analysis.
RESULTS:With the application of microarray, 329 genes were identified as markedly differentially expressed genes between cervical squamous cell carcinoma tissues with and without pelvic lymph node metastasis. Real-time PCR was in accordance with microarray. Forty-four genes associated with tumor metastasis were identified from the differentially expressed gene profile.
CONCLUSION:Gene profile in cervical squamous cell carcinoma tissues associated with pelvic lymph node metastasis is preliminarily identified, indicating that lymph node metastasis of cervical squamous cell carcinoma is a process involving numerous genes.
