Anti-apoptosis and expression of microRNA-21 in rat myocardium during early ischemia-reperfusion injury.
10.3969/j.issn.1672-7347.2013.05.007
- Author:
Qiong YANG
1
;
Kan YANG
;
Anying LI
;
Wenpeng TAN
Author Information
1. Department of Cardiology, Third Xiangya Hospital, Central South University, Changsha 410013,China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
physiology;
Dependovirus;
genetics;
Genetic Vectors;
Male;
MicroRNAs;
genetics;
metabolism;
Myocardial Ischemia;
physiopathology;
Myocardium;
metabolism;
Rats;
Rats, Sprague-Dawley;
Reperfusion Injury;
metabolism;
Transfection
- From:
Journal of Central South University(Medical Sciences)
2013;38(5):483-489
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the expression and anti-apoptosis of microRNA-21(miR-21) in rat myocardium during early ischemia-reperfusion injury (I/R).
METHODS:Sprague-Dawley rats were randomly divided into 5 groups: a control group (transfected with rAAV9-ZsGreen by coronary injection), a miR-21group (transfected rAAV9-ZsGreen-premiR- 21 by coronary injection), a sham group (open-chest only), an I/R group (I/R), and an I/ R+miR-21 (I/R after transfected rAAV9-ZsGreen-pre-miR-21 by coronary injection). Realtime PCR was used to assess the expression level of miR-21. Immunohistochemistry and Western blot were used to determine the expression of Bcl-2, Bax, caspase-3 and Bcl-2/Bax.
RESULTS:MiR-21 was increased by 4.43 times in the miR-21 group (P<0.001). MiR-21 was downregulated in the ischemia zone after I/R compared with the sham group (P<0.05), but that in the non-ischemia zone was significantly increased compared with the sham group (P<0.01). MiR- 21 expression was decreased in the I/R group compared with that in the sham group at 1 h, 2 h and 6 h after I/R (P<0.05), and it was up-regulated in the I/R+miR-21 group at the same time point compared with the I/R group (P<0.01). The expression of Bcl-2, Bax, and caspase-3 was upregulated and Bcl-2/Bax was decreased in the ischemia zone in the I/R group and I/R+miR-21 group than the sham group(P<0.05). Compared with the I/R group, the expression of Bcl-2 and caspase-3 was down-regulated and Bcl-2/Bax was increased in the ischemia zone in the I/ R+miR-21 group (P<0.05).
CONCLUSION:MiR-21 expression is down-regulated and cell apoptosis is increased in rat myocardium during early ischemia-reperfusion injury. Myocardial cell apoptosis may be alleviated by miR-21 over-expression.
