Dynamic changes of cellular HIV-1 DNA quantification during highly active antiretroviral therapy in Chinese HIV infected individuals.
10.3969/j.issn.1672-7347.2013.05.016
- Author:
Xia CHEN
1
;
Yuhuang ZHENG
;
Diallo MAMADOU
;
Yan HE
;
Huaying ZHOU
;
Zi CHEN
;
Yan LUO
;
Bo HE
;
Mei HE
;
Yunhai YAO
Author Information
1. Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha 410008,China.
- Publication Type:Journal Article
- MeSH:
Adult;
Antiretroviral Therapy, Highly Active;
DNA, Viral;
analysis;
Female;
HIV Infections;
drug therapy;
virology;
HIV-1;
genetics;
Humans;
Killer Cells, Natural;
virology;
Male;
Middle Aged;
Monocytes;
virology;
RNA, Viral;
blood;
T-Lymphocytes;
virology;
Viral Load
- From:
Journal of Central South University(Medical Sciences)
2013;38(5):537-544
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the dynamic changes of 3 types of viral reservoir cells (NK cells, T lymphocytes and monocytes), and its relationship with treatment effect in Chinese HIV-1 infected patients receiving highly active antiretroviral treatment (HAART) for 2 years.
METHODS:A total of 40 chronic HIV-1-infected adults who initiated HAART were enrolled in this study and followed up for 2 years. Peripheral whole blood was obtained from each patient at baseline (0 month), 6, 12, 18 and 24 months. Real-time fluorescent quantitative PCR was used to detect the HIV-1 RNA in the plasma and HIV-1 DNA in NK cells, T lymphocytes and monocytes. All the data were statistically analyzed.
RESULTS:CD4 count increased with the decrease of the viral load during HAART. After HAART initiation, HIV-1 DNA showed a significant decrease in NK cells, T lymphocytes and monocytes. The HIV-1 DNA from T lymphocytes, NK cells and monocytes correlated positively with the HIV- 1 RNA (P<0.05) while NK cells and T lymphocytes correlated negatively with CD4+ T cell count. However we did not find significant correlation between CD4+ T cell count and HIV-1 DNA in monocytes at the baseline of HAART.
CONCLUSION:This study found that NK cell was an important HIV cellular reservoir besides T lymphocytes and monocytes. T lymphocytes may be the main long lasting HIV reservoir. HIV-1 proviral DNA may play an important role in the efficacy of treatment and monitoring the disease progression.
