Influence of BRAF interference on SW579 cell lines in thyroid cancer.
10.3969/j.issn.1672-7347.2013.06.005
- Author:
Wenjun YI
1
;
Dewu ZHONG
;
Qiongyan ZOU
Author Information
1. Department of General Surgery, Central South University, Changsha, China. yiwenjun@csco.org.cn
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Humans;
MAP Kinase Signaling System;
Proto-Oncogene Proteins B-raf;
genetics;
metabolism;
RNA Interference;
RNA, Small Interfering;
genetics;
Thyroid Neoplasms;
pathology
- From:
Journal of Central South University(Medical Sciences)
2013;38(6):576-581
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the influence of v-raf murine sarcoma viral oncogene homolog B1 (BRAF) interference on SW579 cell lines in thyroid cancer.
METHODS:We designed 2 pairs of siRNA interference sequences, transfected them into SW579 cell line with liposome, and detected the interference with RT-PCR method. For successfully interfered cell lines, the changes in cell proliferation, cell cycle, and the expression of related proteins in mitogen-activated protein signal-regulated kinase /extracellular signal-regulated kinase (MEK/ ERK) signal pathway were detected.
RESULTS:After 2 pairs of siRNA transfection, the expressions of BRAF mRNA and protein of SW579 cell lines were significantly inhibited (P<0.01). The proliferation was inhibited, the cell cycle was changed, G1/S phase increased, and MEK/ERK signal pathway was inhibited.
CONCLUSION:Inhibition of growth and proliferation of SW579 cell lines by BRAF may be functioned by de-activating MEK/ERK signal pathway.
