Effect of dexmedetomidine hydrochloride on H2O2-induced oxidative stress in alveolar macrophages.
10.3969/j.issn.1672-7347.2013.10.007
- Author:
Lili JIANG
1
;
Zeyou QI
;
Li LI
;
Jinmei SHEN
Author Information
1. Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Line;
Cell Survival;
Dexmedetomidine;
pharmacology;
Hydrogen Peroxide;
L-Lactate Dehydrogenase;
metabolism;
Macrophages, Alveolar;
drug effects;
Oxidative Stress;
Rats;
Receptors, Adrenergic, alpha-2;
metabolism;
Tumor Necrosis Factor-alpha;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2013;38(10):1014-1019
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate whether dexmedetomidine hydrochloride, an α2-adrenergic receptor agonist, can prevent oxidative damage to alveolar macrophages induced by H2O2.
METHODS:We used methyl thiazolyl tetrazolium (MTT) colorimetry to test the effect of different concentrations and action time of H2O2 on the survival rate of alveolar macrophages, and then we chose the appropriate H2O2 concentration and action time to build NR8383 cell oxidative damage model. After pre-conditioning of 0.01, 0.10, and 1.00 μmol/L dexmedetomidine hydrochloride for 24 hours, MTT colorimetry was used to demonstrate the survival rate of NR8383 cells damaged by H2O2, and the release of lactate dehydrogenase (LDH) and TNF-α by H2O2-damaged NR8383 cells was detected by corresponding kit.
RESULTS:At 50-300 μmol/L, H2O2 caused concentration-dependent oxidative damage in the alveolar macrophages, decreased the cell survival rate, and increased LDH and TNF-α release. At 0.01-1.00 μmol/L dexmedetomidine hydrochloride concentration-dependently protected NR8383 cells from oxidative damage induced by H2O2, significantly increased the cell survival rate, decreased LDH and TNF-α release, and this effect of dexmedetomidine hydrochloride was dose-dependent. Yohimbine, an α2 - adrenergic receptor antagonist, completely neutralized the protective effect of dexmedetomidine hydrochloride on NR8383 cells without affecting the oxidative damage of NR8383 cells.
CONCLUSION:Dexmedetomidine hydrochloride can prevent alveolar macrophages from oxidative damage induced by H2O2, which may play a protective role through α2 - adrenergic receptors.
