Identification of contraction related proteins in corpus myometrium at labor.
10.3969/j.issn.1672-7347.2013.10.011
- Author:
Wei MA
1
,
2
;
Changju ZHOU
Author Information
1. Medical School of Northwest University for Nationalities, Lanzhou 730030
2. Department of Obstetrics and Gynecology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Blotting, Western;
Electrophoresis, Gel, Two-Dimensional;
Female;
Humans;
Labor, Obstetric;
physiology;
Mass Spectrometry;
Muscle Contraction;
Muscle Proteins;
analysis;
Myometrium;
physiology;
Pregnancy;
Proteomics;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From:
Journal of Central South University(Medical Sciences)
2013;38(10):1042-1050
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the molecular mechanism of human corpus myometrium contraction related proteins and parturition.
METHODS:The proteins of human corpus of myometrium tissues from full term non-in labor (38- 41 weeks amenorrhea) and full term in labor (38-41 weeks amenorrhea) gravidas were separated by 2-dimensional gel electrophoresis (2-GE), respectively. Then gels were stained by Coomassie brilliant blue G250, scanned by Image scanner and analyzed with PDQuest software. The differentially expressed protein spots of corpus myometrium between the 2 groups were identified by peptide mass finger print based on matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and database searching. Three identified differentially expressed proteins were confirmed by Western blot.
RESULTS:Well resolved and reproducible 2D-GE maps of human corpus myometrium from non-in labor and in-labor gravidas were acquired. Twenty more than 2-fold differentially expressed protein spots were identified by MALDI-TOF-MS. These proteins were involved in cell structure, calcium binding, chaperone, energy metabolism, signal transduction, and antioxidant.
CONCLUSION:Twenty contraction related proteins of human corpus myometrium have been identified, indicating that cell structure and calcium-binding are important reasons for the contraction of human corpus myometrium.
