Identification of pathogenic microorganism by sequencing 16S rRNA gene.
10.3969/j.issn.1672-7347.2013.10.010
- Author:
Feizhou ZHU
1
;
Liyu CHEN
;
Hanchun CHEN
Author Information
1. Department of Biochemistry, School of Life Sciences, School of Basic Medicine, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Bacteria;
classification;
DNA Primers;
DNA, Bacterial;
genetics;
Polymerase Chain Reaction;
RNA, Ribosomal, 16S;
genetics;
Sequence Analysis, DNA
- From:
Journal of Central South University(Medical Sciences)
2013;38(10):1035-1041
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To identify 14 bacteria by sequencing the 16S rRNA gene and establish the basis for clinical application in the future.
METHODS:DNA samples of the 14 bacteria were extracted. The 16S rRNA genes were amplified by PCR and sequenced with common primers. The sequences of the 16S rRNA genes were aligned by online software Blastn in nucleotide database. The bacteria were identified according to the homology of their 16S rRNA genes.
RESULTS:Twelve bacteria were classified to species, the other 2 bacteria were classified to genus.
CONCLUSION:16S rRNA gene sequence analysis is useful in identifying pathogenic bacteria.
