Expression of Notch3 and hypertensive renal fibrosis.
10.3969/j.issn.1672-7347.2013.11.008
- Author:
Hui XU
1
;
Jin ZHANG
;
Li ZHENG
;
Saiya ZHANG
;
Xiaomiao CHENG
Author Information
1. Department of Nephrology, Xiangya Hospital, Central South University, Changsha 410008, China xuhuiye@aliyun.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Arteriosclerosis;
Essential Hypertension;
Fibrosis;
Humans;
Hypertension;
metabolism;
pathology;
Kidney;
pathology;
Kidney Diseases;
metabolism;
pathology;
Rats;
Rats, Inbred SHR;
Rats, Inbred WKY;
Receptor, Notch3;
Receptors, Notch;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2013;38(11):1130-1134
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To examine the expression of notch3 in the kidneys of patients with primary hypertension and rats with spontaneous hypertension, and to explore the relationship of notch3 and hypertension renal fibrosis.
METHODS:Thirteen patients with primary hypertension served as a primary hypertension group (HP group), and 15 patients with kidney tumor served as a control group (CP group). The spontaneous hypertensive rats served as a primary hypertension group (SHR group, n=6), and WKY rats served as a control group (WKY group, n=6). Masson stainning was used to examine the collagen in the kidneys in the SHR group and the WKY group. Immunohistochemical staining was used to detect the levels of Notch3 in kidneys of the patients and the rats. The expression of snail mRNA in the kidneys in the SHR group and the WKY group was examined by real-time PCR.
RESULTS:Masson staining showed much more collagen in the SHR group than that in the WKY group (P<0.05); the expression of Notch3 in the HP group was much higher than that in the CP group ( 6.741±0.231 vs 0.763±0.358, P<0.01). The expression of Notch3 in the SHR group was much higher than that in the WKY group (5.487±0.774 vs 0.421±0.163, P<0.01), and The expression of snail mRNA was much higher in the SHR group than that in the WKY group (0.996±0.120 vs 0.208±0.090, P<0.01 ).
CONCLUSION:Notch3 may be related to the occurrence of hypertension renal fibrosis.
