Regulation mechanism of eIF3 P170 on developing myocardial cell cycle.
10.3969/j.issn.1672-7347.2013.11.011
- Author:
Ting KANG
1
,
2
;
Zuocheng YANG
;
Lihua HUANG
;
Hong XIANG
Author Information
1. Department of Pediatrics, Third Xiangya Hospital, Central South University, Changsha 410013
2. Department of Pediatrics, First affiliated Hospital of South China University, Hengyang Hunan 421000 , China.
- Publication Type:Journal Article
- MeSH:
Animals;
CDC2 Protein Kinase;
metabolism;
Cell Cycle;
Cyclin B1;
metabolism;
Cyclin D1;
metabolism;
Eukaryotic Initiation Factor-3;
metabolism;
Mice;
Myocytes, Cardiac;
cytology;
metabolism;
RNA, Messenger
- From:
Journal of Central South University(Medical Sciences)
2013;38(11):1146-1151
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of eIF3P170, cdc2, cyclinB1 and cyclinD1 in developing cardiac myocytes, and the correlation between eIF3P170 with cdc2, cyclin D1, and cyclin B1 in mice.
METHODS:Mouse cardiac myocytes were obtained at different time points. RT-PCR was employed to detect the expression of eIF3P170, cdc2, cyclin D1 and cyclin B1 mRNA.
RESULTS:Expressions of eIF3P170, cdc2, cyclinD1 and cyclinB1 mRNA were higher in the embryonic Day 13, 15, 18 and postnatal Day 1, 2, 3, 5. Expressions at postnatal Day 5 reached the highest (all P values<0.05 vs other time points), and then the expressions of these genes gradually decreased to the weakest at postnatal Day 30 (all P values<0.05 vs other time points). The mRNA expression of eIF3P170 was positively correlated with cdc2, cyclin D1 and cyclin B1 mRNA expression respectively.
CONCLUSION:The mRNA expressions of eIF3 P170, cdc2, cyclin D1 and cyclin B1 in the embryo and the early life after birth are high. They reach the maximum at postnatal Day 5, then gradually decreased.
