Effect of ropivacaine on proliferation and migration of rat bone marrow mesenchymal stem cells.
10.3969/j.issn.1672-7347.2013.11.012
- Author:
Xiaoshu GUO
1
;
Jiping GONG
;
Genqing YANG
;
Yongli CHANG
;
Lijing GAO
;
Xiaoxia TIAN
Author Information
1. Department of Physiology, Changzhi Medical College, Changzhi Shanxi 046000, China guoxiaoshu999@163.com.
- Publication Type:Journal Article
- MeSH:
Amides;
pharmacology;
Animals;
Bone Marrow Cells;
Cell Differentiation;
Cell Movement;
drug effects;
Cell Proliferation;
drug effects;
Cells, Cultured;
Flow Cytometry;
Mesenchymal Stem Cells;
cytology;
drug effects;
Rats;
Ropivacaine
- From:
Journal of Central South University(Medical Sciences)
2013;38(11):1152-1159
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the influence of ropivacaine on the proliferation and migration of rat bone marrow mesenchymal stem cells (BMSCs) and provide basis for the clinical application of BMSCs.
METHODS:Rat BMSCs were isolated and cultured by adherence method. Surface markers of BMSCs were examined by flow cytometry. Multipotent differentiation of BMSCs was detected by induced adipogenesis, osteogenesis and muscular differentiation. Proliferation of BMSCs was examined by CCK-8 and Brdu incorporation after ropivacaine treatment at different concentrations. Migration of BMSCs was tested by cell scratch assay and Millicell experiment.
RESULTS:Cultured cells had representative appearance and surface markers of BMSC, and they had potential multiple differentiation. Ropivacaine treatment at 50 and 100 μmol/L significantly reduced the proliferation rate of BMSCs and Brdu incorporation rate. There was significant difference compared with the control group (P<0.05). Cellular scratch assay and migration experiment indicated that ropivacaine significantly reduced the migration of BMSCs. There was significant difference compared with the control group (P<0.05). All these mentioned effects of ropivacaine on BMSCs were dose-dependent. There was significant difference between groups (P<0.05).
CONCLUSION:Ropivacaine can significantly reduce the proliferation and migration of rat BMSCs, suggesting that the influence of local anesthetics on BMSCs has to be taken into account when BMSCs are used in clinical practice.
