Ultraviolet radiation-induced apoptosis in human lens epithelial cells and its effect on Bcl-2 and Bax.
10.3969/j.issn.1672-7347.2012.07.015
- Author:
Songbai JIA
1
;
Jingming SHI
;
Xuan CHEN
;
Luosheng TANG
Author Information
1. Department of Ophthalmology, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
radiation effects;
Cells, Cultured;
Epithelial Cells;
cytology;
metabolism;
radiation effects;
Humans;
Lens, Crystalline;
cytology;
radiation effects;
Proto-Oncogene Proteins c-bcl-2;
genetics;
metabolism;
RNA, Messenger;
genetics;
metabolism;
Time Factors;
Ultraviolet Rays;
adverse effects;
bcl-2-Associated X Protein;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2012;37(7):730-736
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the apoptosis-inducing effect of ultraviolet(UV) radiation on human lens epithelial cells (HLEC), with particular focus on changes in Bcl-2 or Bax expression as possible mechanisms.
METHODS:All experimental groups were exposed to the same UV light source. HLEC were divided into 6 groups according to duration of UV radiation : 0 min group (control group), 5 min group, 10 min group,15 min group, and 30 min group. Analysis on apoptosis of HLEC was performed by flow cytometry analysis (FCA, Annexin V + PI staining). Changes of Bax and Bcl-2 expression in HLEC were detected by hybridization in situ.
RESULTS:Apoptosis in HLEC increased with UV exposure time. The expression level of Bax mRNA was increased with the increase of UV exposure time, whereas the expression level of Bcl-2 mRNA decreased with the increase of UV exposure time. The proportion of apoptotic cells was negatively correlated with ratio of Bcl-2/Bax (r=-0.874, P<0.05).
CONCLUSION:UA radiation can induce apoptosis of HLEC in vitro. Bcl-2 and Bax genes may play an important role in regulating this apoptotic process.
