MK801 controls form-deprivation myopia by nitric oxide-cyclic GMP signaling pathway in guinea pig.
10.3969/j.issn.1672-7347.2012.07.016
- Author:
Dan WEN
1
;
Shuangzhen LIU
;
Junfeng MAO
;
Xingping TAN
;
Chaohua XIA
;
Chunan YIN
Author Information
1. Department of Ophthalmology, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cyclic GMP;
metabolism;
Dizocilpine Maleate;
administration & dosage;
pharmacology;
Down-Regulation;
Female;
Form Perception;
physiology;
Guinea Pigs;
Injections, Intraocular;
Male;
Myopia;
physiopathology;
Nitric Oxide Synthase Type I;
metabolism;
Sensory Deprivation;
physiology;
Signal Transduction;
drug effects;
Vitreous Body;
drug effects
- From:
Journal of Central South University(Medical Sciences)
2012;37(7):737-742
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the mechanism of myopia following intravitreous injection of MK801 (dizocipine maleate) intravitreous injected.
METHODS:Three-week-old guinea pigs were divided into six groups: group A (control), group B (3 weeks form-deprivation in right eye), group C ( 3 weeks form-deprivation in right eye + saline), group D (3 weeks form-deprivation in right eye + MK801 1ng), group E (3 weeks form-deprivation in right eye + MK801 10 ng), group F (3 weeks form-deprivation in right eye + MK801 100 ng). The refraction and axial length of the eyes were measured. ncNOS was measured by hybridization in situ, and cyclic GMP (cGMP) concentrations by radioimmunochemistry. The correlation between MK801 concentration and diopter degree, axial length of the eyes, and levels of ncNOS or cyclic GMP were analyzed with linear correlation in the groups C-F.
RESULTS:Diopter degree was decreased, axial eye length was shorted and levels of ncNOS and c-GMP were decreased in groups C, D, E and F dependent on the concentration of MK801. The diopter degree had positive correlation with MK801 concentration (r=0.702, P<0.05), while the axial eye length and the levels of ncNOS and cGMP were negatively correlated (r=-0.736, -0.637, -0.725, P<0.05)
CONCLUSION:MK801 injected into the vitreous humor can restrain myopia by down-regulated the expression of the nitric oxide-cyclic GMP signaling pathway. The effect is concentration dependent.
