IL-10 suppresses inflammatory granuloma uptake of 18F-FDG in rats.
10.3969/j.issn.1672-7347.2012.07.018
- Author:
Jianjun HE
1
;
Qianchun YE
;
Ke ZHI
Author Information
1. Department of Nuclear Medicine, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Female;
Fluorodeoxyglucose F18;
pharmacokinetics;
Freund's Adjuvant;
Granuloma;
chemically induced;
metabolism;
Interleukin-10;
pharmacology;
Lung Diseases;
chemically induced;
metabolism;
Male;
Radiopharmaceuticals;
pharmacokinetics;
Rats;
Serum Albumin, Bovine
- From:
Journal of Central South University(Medical Sciences)
2012;37(7):748-751
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the effectiveness of IL-10 in suppressing 18F-FDG uptake in the inflammatory granuloma of SD rats.
METHODS:Eight SD rats were killed, and their blood was collected sterily. After centrifugion, the white blood cells were incubated in PRMI 1640 for 3 days. Then each culture flask of white blood cells was divided into two equal parts. To one group was added 0.2 mL IL-10 solution (0.1 mg/mL); to the control was added with 0.2 mL of 0.9% sodium chloride solution. All cells were then incubated for 120 minutes at 37 degree, after which 18F-FDG (1.85 MBq) was added. Sixty minutes later, the cells were washed twice with PBS and the extent of uptake 18F-FDG determined. In vivo, an inflammatory granuloma was produced by hypodermic injection of rats with a mixture of Freund's complete adjuvant, bovine serum albumin and talcum powder. Each rat was maintained for 8 weeks. Imaging of the inflammatory granulomas was performed using the 18F-FDG signal. IL-10 was injected into SD rats at 10 μg/kg of body weight. Sixty minutes later, 7.4 MBq of 18F-FDG were injected, and, after a further 60 minutes, the rats underwent a PET-CT scan. The region of interest (ROI) of the inflammatory granuloma was delineated and the standard uptake value (SUV) calculated. A second PET-CT scan was done without IL-10 on the next day. The granulomatous tissue underwent pathological examination.
RESULTS:In the intro test, the with blood cell uptaking ratio of 18F-FDG was (50.3±6.7)% without IL-10, and (34.6±3.5)% with IL-10(t=8.9, P<0.01). IL-10 suppressed the rat white blood cell uptaking 18F-FDG. In the PET-CT scan, the SUV of ROI on inflammatory granuloma was 1.7±0.4 with IL-10 and 2.1±0.3 without IL-10 (t=20.6, P<0.01). IL-10 suppressed the inflammatory granuloma uptaking 18F-FDG.
CONCLUSION:IL-10 can suppress the inflammatory granuloma of SD rats uptaking 18F-FDG.
