Protective effect of SjC23-Hsp70 DNA vaccine and interleukin-12 on Schistosoma japonicum infection in water buffalos.
10.3969/j.issn.1672-7347.2012.08.017
- Author:
Pingcheng HU
1
;
Da XIA
;
Hongyan CUI
;
Pingfang ZHANG
;
Yongkang HE
;
Xinling YU
;
Zhenqiu SUN
Author Information
1. Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha 410078,China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antigens, Helminth;
immunology;
Buffaloes;
Cattle;
HSP70 Heat-Shock Proteins;
genetics;
immunology;
Helminth Proteins;
immunology;
Immunization;
methods;
Interleukin-12;
genetics;
immunology;
Membrane Proteins;
immunology;
Schistosomiasis japonica;
immunology;
prevention & control;
veterinary;
Vaccines, DNA;
administration & dosage;
immunology;
Vaccines, Synthetic;
immunology
- From:
Journal of Central South University(Medical Sciences)
2012;37(8):854-859
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the immune-protective effect of Japan Schistosoma (Chinese mainland strain) 23 kD membrane protein-heat shock protein (SjC23-Hsp70) DNA vaccine plus adjuvantinduced interleukin-12 (IL-12) plasmid DNA on Schistosoma japonicum infection in water buffalos.
METHODS:Forty-five health water buffalos (8-10 months old) in non-endemic area of schistosomiasis were randomly assigned into group A (SjC23-Hsp70+IL-12, 300 μg), group B (SjC23+IL-12, 300 μg) and group C (pVAX+IL-12, 300 μg), 15 in each group. Each buffalo was immuned by shoulder intramuscular injection for 3 times, at an interval of 28 days. Twenty-eight days after the last immunization, each buffalo was infected with 1000 Japan cercariae of Schistosoma. Fecal examinations were conducted 2 days and 1 day before the perfusion, and on the day of perfusion. The number of hatching miracidia and eggs per gram feces was recorded. Fifty-six days after the infection, the buffalos were sacrificed and perfused via the descending aorta. The recovered adult worms and eggs in the liver tissue were counted.
RESULTS:We compared group A and B with group C: the estrogen reduction rate was 45.7% and 26.61%; bug reduction rate was 44.51% and 25.84%; the fecal egg reduction rate was 41.1% and 31.63%; the miracidium reduction rate was 48.11% and 38.07%; and the liver egg reduction rate was 43.39% and 31.95%. The above rates in group A were higher than those in group B (P<0.05).
CONCLUSION:SjC23-Hsp70 DNA vaccine combined with IL-12 may have a significant immunoprotective effect on buffalos.
