Effect of gallnut extract on nasopharyngeal carcinoma 5-8F cells and its mechanism.
- Author:
Qiu XIANG
1
;
Caiwen FAN
;
Shengjun XIAO
;
Hongjie PAN
;
Jianhong WANG
;
Ning ZHAO
;
Jing TIAN
Author Information
1. Department of Pathology and Pathophysiology, Guilin Medical College, Guilin Guangxi, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents, Phytogenic;
pharmacology;
Apoptosis;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Cyclooxygenase 2;
genetics;
metabolism;
Down-Regulation;
drug effects;
Ellagic Acid;
pharmacology;
Humans;
Nasopharyngeal Neoplasms;
pathology;
Plant Extracts;
pharmacology;
Stathmin;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2012;37(9):871-875
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the biological activity of ellagic acid extracted from gallnut against nasopharyngeal carcinoma and its molecular mechanism.
METHODS:Nasopharyngeal carcinoma 5-8F cells were treated with 2, 4, 6 μg/mL ellagic acid for 48 h in vitro. The cell proliferation and cell apoptosis were analyzed by MTT and Hoechst33258 stain. The cell cycle and protein expression were measured by flow cytometry and Western blot.
RESULTS:Ellagic acid inhibited the proliferation of 5-8F cells. The inhibition rates were (29.35±4.95)%, (53.32 ±4.44)% and ( 61.75 + 6.93)%, respectively, with significant difference from the control group (P<0.01). S phase cells in the experimental groups were (25.47±0.74)%, (28.08±1.41)% and (35.49±0.66)%, respectively, with significant difference (P<0.01) from the control group (21.26±0.70)%. Cells in the experimental groups showed nuclear pyknosis, karyorrhexis and poptotic cell morphology. The expression of COX-2 and stathmin in 5-8F cells was down-regulated with increased drug concentration.
CONCLUSION:Ellagic acid extracted from gallnut has activity against nasopharyngeal carcinoma cells, and its mechanism may be related to down-regulated expression of COX-2 and stathmin.
