hMLH1 gene promoter methylation in joint cartilage in patients with osteoarthritis.
- Author:
Min XU
1
;
Yi SHEN
;
Qiang ZHANG
;
Minghui CHEN
;
Bo YU
;
Dan PENG
Author Information
1. Department of Orthopedics, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Adaptor Proteins, Signal Transducing;
genetics;
metabolism;
Adolescent;
Adult;
Cartilage, Articular;
metabolism;
Child;
DNA Methylation;
Female;
Humans;
Male;
MutL Protein Homolog 1;
Nuclear Proteins;
genetics;
metabolism;
Osteoarthritis;
genetics;
metabolism;
Promoter Regions, Genetic;
genetics;
Young Adult
- From:
Journal of Central South University(Medical Sciences)
2012;37(9):928-933
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the role of Human MutL homologue 1 (hMLH1) gene promoter methylation in the occurrence and development of osteoarthritis (OA).
METHODS:General DNA was dealt with sodium bisulfite. The methylation of hMLH1 promoter was detected by methylation-specific PCR (MSP). hMLH1 protein expression in joint cartilage was detected by immunohistochemical method.
RESULTS:The positive percent of hMLH1 promoter methylation in OA patients was higher than that in healthy persons (χ(2)=30.634, P<0.001); the positive percent of hMLH1 protein in OA patients was significantly lower than that in healthy persons (χ(2)=37.724, P<0.001); prmoter methylation and protein expression level of hMLH1 gene showed negative correlation (rs=-0.554, P<0.001).
CONCLUSION:hMLH1 promoter is hypermethlated in joint cartilage cells of OA patients. Hypermethylation may affect the protein expression of hMLH1, which might play a role in the occurrence and development of OA.
