Rapid detection of microalbuminuria with high-performance liquid chromatography.
10.3969/j.issn.1672-7347.2012.10.017
- Author:
Fang LIU
1
;
Yueyun XIANG
;
Keqian XU
Author Information
1. Department of Clinical Laboratory, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Albuminuria;
diagnosis;
Chromatography, High Pressure Liquid;
Diabetes Mellitus;
urine;
Humans
- From:
Journal of Central South University(Medical Sciences)
2012;37(10):1066-1070
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a rapid detection method for microalbuminuria with size-exclusion high performance liquid chromatography.
METHODS:With a new mobile phase, samples were injected onto an Agilent Zorbax GF-250 size exclusion chromatographic column. The method was evaluated and urine albumin of 56 diabetic patients was analyzed.
RESULTS:The mobile phase containing 0.1% formic acid and acetonitrile, with 20 μL sample size, 1 mL/min flow rate, 205 nm detection wavelength. The retention time of albumin both in human serum and urine was 1.7 min. The linear range was 5-2000 mg/L. The lower limit of measurement was 2 mg/L. The intra-assay coefficient of variation and the inter-assay coefficient of variation were 3.98% and 4.05% (20 mg/L), 3.55% and 3.60% (200 mg/L), 4.65% and 4.74% (2000 mg/L), respectively. Recovery rates were 95.3%, 98.1%, and 97.2%. Microalbuminuria was detected in 30 samples by high performance liquid chromatography and 15 samples by immunoturbidimetry from 56 patients with diabetic mellitus.
CONCLUSION:A fast and high sensitivity method, namely size-exclusion high performance liquid chromatography, with mobile phase containing 0.1% formic acid and acetonitrile has been established to analyze microalbuminuria, which can detect more microalbuminuria than other methods and is suitable for clinical routine measurement.
