Effect of calcitonin gene-related peptide on the maturation of oocyte in vitro.
10.3969/j.issn.1672-7347.2012.10.001
- Author:
Zhe ZHANG
1
;
Hongqing LIAO
;
Shuoping ZHANG
;
Xiaoming XU
;
Fei GONG
;
Guangxiu LU
Author Information
1. Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Calcitonin Gene-Related Peptide;
pharmacology;
Cyclic AMP;
metabolism;
Female;
Granulosa Cells;
cytology;
Humans;
In Vitro Techniques;
Mice;
Oocytes;
cytology;
drug effects
- From:
Journal of Central South University(Medical Sciences)
2012;37(10):973-978
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of calcitonin gene-related peptide (CGRP) on murine oocyte maturation.
METHODS:After injection of pregnant mare serum gonadotropin (PMSG, 10 U, i.p.) for 48 h, 6-week old female Kunming mice were killed, and the cumulus oocyte complexes (COCs) were collected from ovaries and inoculated in the culture plate by 30-40/hole. The COCs were treated with 4 concentrations of CGRP (0, 10(-10), 10(-9), and 10(-8) mol/L), and the germinal vesicle breakdown (GVBD) and polar body I (PBI) were examined. Human granulosa cells were also cultured with CGRP (0, 10(-10), 10(-9), 10(-8) mol/L) and levels of intracellular cyclic adenosine monophosphate (cAMP) were measured.
RESULTS:Exogenous CGRP caused a decrease in GVBD and PBI in COCs, and an increase in cAMP levels in human granulosa cells in a concentration-dependent manner.
CONCLUSION:CGRP can inhibit the oocyte maturation, which may be related to the increased content of cAMP in granulosa cells.
