Gender related metabonomic analysis of serum and urine samples from Xinjiang healthy Han subjects with magnetic resonance.
10.3969/j.issn.1672-7347.2012.11.010
- Author:
Batur MAMTIMIN
1
;
Qianghui MIAN
;
Chunli CHEN
;
Ayshamgul HASIM
;
Li YOU
Author Information
1. College of Pharmacy, Xinjiang Medical University, Urumqi 830011, China. mtbatu@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Amino Acids;
blood;
urine;
Blood Chemical Analysis;
China;
ethnology;
Citric Acid;
blood;
urine;
Creatinine;
blood;
urine;
Female;
Humans;
Lipids;
blood;
urine;
Lipoproteins;
blood;
urine;
Magnetic Resonance Spectroscopy;
Male;
Metabolome;
Metabolomics;
methods;
Sex Factors;
Urinalysis
- From:
Journal of Central South University(Medical Sciences)
2012;37(11):1135-1140
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate gender variability in the metabolic serum and urinary profile of healthy Han population in Xinjiang.
METHODS:Serum and urinary samples from 92 healthy Han people in Xinjiang were tested by magnetic resonance based metabonomics and pattern recognition analysis performed with orthogonal partial least-squares discriminant analysis (OPLS-DA). The quality of the model was described by parameter R(2)X, R(2)Y, and Q(2).
RESULTS:The serum in males had higher levels of very low density lipoprotein, low density lipoprotein, unsaturated lipids, creatinine and acetone than in females, whereas females had higher levels of citrate, choline, glucose and amino acids (including isoleucine, leucine, valine, alanine, citrulline, lysine, methionine, glutamate, phenylalanine, threonine, tyrosine, 1-methyl histidine and glycine) than in males. The urine of males had higher levels of formate, malonic acid, taurine, creatinine than that of females, while females had higher levels of hippurate, γ-aminobutyric acid, succinate, citrate and glutamate than males. The model parameters of serum were R(2)X=0.64, R(2)Y=0.70, and Q(2)=0.67, and those of urine were R(2)X=0.17, R(2)Y=0.70, and Q(2)=0.44.
CONCLUSION:The blood and urine from Han population in Xinjiang contain a variety of gender related metabolites, which plays an important role in the research of clinical metabonomics.
