Comparison of growth of human fetal RPE cells on electrospun nanofibers and etched pore polyester membranes.
10.3969/j.issn.1672-7347.2012.05.001
- Author:
Yunping LI
1
;
Luosheng TANG
Author Information
1. Department of Ophthalmology, Second Xiangya Hospital, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Biocompatible Materials;
chemistry;
Bruch Membrane;
Cell Proliferation;
Cells, Cultured;
Fetus;
Humans;
Membranes, Artificial;
Nanofibers;
chemistry;
Polyesters;
chemistry;
Porosity;
Rats;
Retinal Pigment Epithelium;
cytology;
growth & development;
Tissue Engineering
- From:
Journal of Central South University(Medical Sciences)
2012;37(5):433-440
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate and compare the growth of human fetal retinal pigment epithelial (RPE) cells seeded onto electrospun polyamide nanofibers (EPN) or etched pore polyester (EPP), and, further, to explore their possible use as prosthetic Bruch's membrane.
METHODS:Human fetal RPE cells were planted onto the EPN, EPP and plastic (control) substrates in Transwells. The cultures were assessed with respect to cell attachment at 2, 4, 8 hours and proliferation at 1, 4, 8 days after seeding. Growth and morphology of the cells were monitored under the phase contrast microscope, and the phenotype was identified by immunofluorescence staining with antibodies against tight junction protein ZO-1. Strips of single EPP coated with nothing or EPP coated with EPN was differently implanted into the subretinal space of two P21 RCS rats for two weeks and the histologic slides of the retina were assessed.
RESULTS:Cultured human fetal RPE cells were attached to either EPN or EPP substrates (with seeding on plastic substrate as control). After 8 h, the numbers of adherent cells in the EPN, EPP and control groups were 1.23*10(5)/cm(2), 1.70*10(5)/cm(2), and 1.64*10(5)/cm(2), respectively. The number of RPE cells attached to EPN was obviously less than that to both EPP and control (P<0.05). On the first day, the proliferation of cells on EPN was less than that of EPP and control (P<0.05); but by the 8th day in culture, the proliferation of cells on EPN had increased and was higher than proliferation on both EPP and control (P<0.05). All of the RPE cells cultured on EPN and EPP substrates were in monolayer, and the EPN-attached cells resembled the inner collagenous layer of Bruch's membrane. Immunofluorescence staining showed that the RPE cells cultured on EPN and EPP substrates adopted a higher expression of ZO-1 than that on the plastic control substrate. Subretinal implantation of either EPP alone or EPP as a carrier for free EPN for 2 weeks in P21RCS rats resulted in an expected encapsulation and loss of photoreceptor layer. No toxicity or other adverse reaction was observed in the vicinity of the transplant.
CONCLUSION:EPN and EPP could maintain human fetal RPE cell attachment and proliferation. Both EPN and EPP appeared to be grossly tolerance and biocompatible with subretinal implantation. EPN represents an intriguing prospect for prosthetic Bruch's membrane replacement because of its similarity in structure to native Bruch's membrane.
