Effect of ultraviolet radiation on ALDH1 expression in human lens epithelial cells.
10.3969/j.issn.1672-7347.2012.06.006
- Author:
Jingming SHI
1
;
Songbai JIA
;
Xuan CHEN
;
Luosheng TANG
Author Information
1. Department of Ophthalmology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Aldehyde Dehydrogenase 1;
Apoptosis;
radiation effects;
Cells, Cultured;
Epithelial Cells;
cytology;
metabolism;
radiation effects;
Humans;
Isoenzymes;
genetics;
metabolism;
Lens, Crystalline;
cytology;
Retinal Dehydrogenase;
genetics;
metabolism;
Ultraviolet Rays;
adverse effects
- From:
Journal of Central South University(Medical Sciences)
2012;37(6):572-577
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the apoptosis-inducing effect of ultraviolet light (UV) on human lens epithelial cell (HLEC) and to explore the involvement of changes in ALDH1 folowing UV radiation.
METHODS:HLEC was exposed to the same UV light source and was subsequently divided into 6 groups according to UV radiation time of 0 (control group), 5, 10, 15, and 30 min. Apoptosis was detected by AO/EB staining. Changes of ALDH1 in HLEC were detected by immunohistochemical staining and Western blot.
RESULTS:The intensity of immunohistochemical staining and the rate of positive cells decreased with increase of UV time (P<0.05). The rate of positive ALDH1 cells was negatively correlated with the rate of apoptosis (r= -0.92, P<0.05). Western blot showed the integrated absorbance values significantly decreased with the increase of UV time (P<0.05).
CONCLUSION:ALDH1 in HLEC decreases with an increase of UV exposure, which may be related to UV induced apoptosis of HLEC.
