MYETS1 recombinant expression in prokaryotic cells and deletion analysis in multiple myeloma cell lines.
10.3969/j.issn.1672-7347.2012.01.005
- Author:
Jianjun WANG
1
;
Liping HONG
;
Yi PAN
;
Shuiping LIU
;
Kunlu WU
;
Lijun TANG
Author Information
1. Institute of Life Science and Technology, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Chromosomes, Human, Pair 13;
genetics;
Gene Deletion;
Genetic Vectors;
genetics;
Humans;
Intercellular Signaling Peptides and Proteins;
biosynthesis;
genetics;
Membrane Proteins;
biosynthesis;
genetics;
Multiple Myeloma;
genetics;
metabolism;
pathology;
Neoplasm Proteins;
biosynthesis;
genetics;
Recombinant Proteins;
biosynthesis;
genetics
- From:
Journal of Central South University(Medical Sciences)
2012;37(1):27-31
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the down-expression mechanism of MYETS1 gene in multiple myeloma cell lines ARH-77 or KM3, and express MYETS1 gene in prokaryotic express system.
METHODS:The region of chromosome 13q14.3 in ARH-77 and KM3 was detected by FISH. MYETS1 gene was amplified by RT-PCR and cloned into prokaryotic expression vector pGEX-4T.
RESULTS:Positive consequence was acquired in 13q14.3 where MYETS1 located by FISH in ARH- 77 and KM3 cell lines. Bioinformatics indicated highly sequence homology between MYETS1 and LECT1, but excluded the homology of open reading frame between MYETS1 and that of LECT1 by RT-PCR. Myets1 protein was expressed and harvested successfully.
CONCLUSION:The region of chromosome 13q14.3 ,where MYETS1 gene located, was not defected in ARH-77 and KM3 cell lines. Down-expression of MYETS1 might be regulated by other mechanisms in multiple myeloma cell lines.
