Effect of C23 down-regulation on proliferation inhibition and apoptosis induced by cisplatin in human osteosarcoma SaOS-2 cells.
10.3969/j.issn.1672-7347.2012.01.006
- Author:
Bei WU
1
;
Wanchun WANG
;
Zhihong LI
Author Information
1. Department of Orthopedics, Second Xiangya Hospital, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Apoptosis;
drug effects;
Bone Neoplasms;
metabolism;
pathology;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Cisplatin;
pharmacology;
Down-Regulation;
Humans;
Osteosarcoma;
metabolism;
pathology;
Phosphoproteins;
metabolism;
RNA-Binding Proteins;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2012;37(1):32-37
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of C23 down-regulation on proliferation inhibition and apoptosis induced by cisplatin in human osteosarcoma SaOS-2 cells.
METHODS:SaOS-2 cells were randomly divided in to 5 groups: a control group, a DDP group, an S+DDP group, an AS+DDP group, and an R+DDP group. Cell growth suppression of each group was analyzed by MTT assay. RT-PCR and Western blot were used to detect the expression of C23, Bcl-2 and Bax mRNA and protein in each group. Morphologic character of apoptosis was evaluated by DAPI staining. The percentage of apoptotic cells was analyzed by flowcytometer assay.
RESULTS:C23 antisense oligonucleotides remarkably inhibited the expression of C23 mRNA and protein in SaOS-2 cells (P<0.01). The proliferation of SaOS-2 cells was remarkably inhibited in the AS+DDP group compared with the DDP group (P<0.01). The expression of C23, Bcl-2 mRNA and protein was weaker and that of Bax mRNA and protein was stronger in the AS+DDP group compared with the DDP group (P<0.01). Cisplatin-mediated apoptosis neclei in SaOS-2 cells and the rate of apoptotic cells were higher in the AS+DDP group than those of the DDP group (P<0.01).
CONCLUSION:Down-regulation of C23 can contribute to cisplatin-mediated proliferation inhibition and apoptosis in SaOS-2 cells.
