Effect of extracts from Radix Trichosanthis on the expression of HBsAg and HBeAg in HepG2.2.15 cells.
10.3969/j.issn.1672-7347.2012.01.007
- Author:
Jia CHEN
1
;
Zhaofa XU
;
Hongtao OUYANG
;
Minyuan PENG
;
Ting WU
;
Jing LIU
;
Yanping LIU
;
Huiwen YAN
Author Information
1. Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Antiviral Agents;
pharmacology;
Drugs, Chinese Herbal;
classification;
pharmacology;
Hep G2 Cells;
Hepatitis B Surface Antigens;
biosynthesis;
Hepatitis B e Antigens;
biosynthesis;
Hepatitis B virus;
drug effects;
Humans
- From:
Journal of Central South University(Medical Sciences)
2012;37(1):38-41
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of extracts with water and alcohol from Radix Trichosanthis on the cell survival and the expression of hepatitis B surface antigen (HBsAg) and hepatitis B e-antigen (HBeAg) in HepG2.2.15 cell supernatant.
METHODS:The cell survival rate of HepG2.2.15 cells was detected by MTT assay. The HBsAg and HBeAg in HepG 2.2.15 cell supernatant were evaluated by enzyme linked immunosorbent assay.
RESULTS:The water and alcohol soluble extracts from Radix Trichosanthis significantly inhibited the levels of HBsAg and HBeAg in HepG2.2.15 cells in a time-and-concentration-dependent manner. However, the therapeutic index of extracts with water from Radix Trichosanthis was better than that in the alcohol group.
CONCLUSION:The activity of water-soluble extract from Radix Trichosanthis is stronger on anti-hepatitis B virus than that of the alcohol-soluble extract.
