Differential expression of taxol resistance and taxol resistance reversal related genes in nasopharyngeal carcinoma by cDNA microarray.
10.3969/j.issn.1672-7347.2012.01.009
- Author:
Xiaowei PENG
1
;
Guolin TAN
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors;
genetics;
Cell Line, Tumor;
Cytochrome P-450 CYP1A1;
genetics;
Drug Resistance, Neoplasm;
genetics;
Gene Expression Regulation, Neoplastic;
Humans;
Nasopharyngeal Neoplasms;
pathology;
Oligonucleotide Array Sequence Analysis;
Paclitaxel;
pharmacology;
Proteins;
genetics;
Receptors, Tumor Necrosis Factor;
genetics;
TWEAK Receptor
- From:
Journal of Central South University(Medical Sciences)
2012;37(1):48-52
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To compare the difference in gene expression profiles between parental cell line and drug resistant cell line (CNE-1 and CNE-1/taxol) pre-treated or treated by drugs, and search for genes related to taxol resistance and reversal of taxol resistance phenotype.
METHODS:cDNA microarray was used to detect the difference in gene expression profiles between 6 groups of cells. Combination of multiple filtering genes and detailed analysis of documented resistance genes were used to analyze the data.
RESULTS:Through multiple filtering, 297 differentially expressed genes were screened. The expression of 17 genes was increased or decreased more than 5 folds in CNE-1/taxol compared with CNE-1.Through analyzing documented drug-resistant genes, MDR1 expression was not detected in each group. CYP1A1, one of P450 family members, was not expressed in CNE-1, but significantly increased expressions was found in CNE-1/taxol and these increased expressions were restored by cisplatin. The expression level of some members of tumor necrosis factor family was decreased in CNE-1/taxol and restored by cisplatin, including TNFAIP1, 3 and TNFRSF12A, 21. The differentially expressed members in the caspase family were caspase-4 and caspase-6. The expression of β-tubulin II was down-regulated in CNE-1/taxol. TSP1 was obviously down-regulated in CNE- 1/taxol compared with CNE-1, and a more significant down-regulation of TSP1 was found when treated by taxol. However, it was greatly up-regulated after cisplatin treatment in CNE-1/taxol.
CONCLUSION:Some genes are probably related to taxol resistance and reversal of taxol resistance in NPC cells: 297 differentially expressed genes detected by multiple filing, CYP1A1, some members of TNF family and another 17 genes whose differential expression is more than 5 folds between parental cell line and drug resistant cell line. Combination of multiple filtering genes and detailed analysis of documented resistance genes is a good method to study drug resistance and reversal of drug resistance in carcinoma cells.
