Proapoptotic effect of angiotensin II on renal tubular epithelial cells and protective effect of Cordyceps sinensis.
10.3969/j.issn.1672-7347.2012.01.012
- Author:
Shan TU
1
;
Qiaoling ZHOU
;
Rong TANG
;
Tianfeng TANG
;
Sai HU
;
Xiang AO
Author Information
1. Department of Nephrology, Xiangya Hospital, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Angiotensin II;
pharmacology;
Apoptosis;
drug effects;
Caspase 3;
metabolism;
Cell Line;
Cells, Cultured;
Cordyceps;
chemistry;
Drugs, Chinese Herbal;
pharmacology;
Epithelial Cells;
cytology;
Humans;
Kidney Tubules;
cytology;
Protective Agents;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2012;37(1):67-72
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the mechanism of the protective effect of Cordyceps sinensis (C. sinensis) on the apoptosis of cultured NRK-52E induced by angiotension II (AngII).
METHODS:NRK-52E cells were incubated with C. sinensis (0, 5, 10, 20, and 40 mg/L) and 10(-8) mol/ L AngII for 24, 48, 72 h. The optimal concentration of C. sinensis was selected. Either NRK-52E cells were incubated with different doses of AngII (0, 10(-12), 10(-10), 10(-8), and 10(-6) mol/L) for 24 h, or with 10(-8) mol/L AngII for 24, 48, and 72 h, to observe the effect of AngII on the apoptosis of NRK- 52E cells. The optimal concentration and time of AngII were selected. In another experiment cells were divided into 5 groups: a control, AngII (10(-8) mol/L), AngII (10(-8) mol/L)+ C. sinensis (40 mg/ L), Ang II (10(-8) mol/L)+ fosinopril (10(-5) mmol/L), and Ang II (10(-8) mol/L)+ fosinopril (10(-5) mol/ L)+C. sinensis (40 mg/L). MTT assay was used to test the changes in the proliferation of NRK-52E cultured with different concentration of C. sinensis for 24, 48, 72 h. The Annecxin V-FITC and PI stainings were applied to detect the apoptosis rate induced by AngII by flow cytometer (FCM) and to determine the eddects of C. sinensis. The activity of caspase-3 was assayed by spectrophotometry.
RESULTS:Certain concentrations of C. sinensis (10-40 mg/L) promoted the proliferation of NRK- 52E cells inhibited by AngII(P<0.05). AngII induced the apoptosis of NRK-52E in a dose and timedependent manner, accompanied with increased activity of caspase-3 (P<0.05). C. sinensis partially suppressed the apoptosis of NRK-52E induced by AngII, and declined the activity of caspase-3 (P<0.05). No significant difference was shown as between the fosinopril group and the fosinopril+C. sinensis group (P>0.05).
CONCLUSION:C. sinensis can suppress the apoptosis of NRK-52E by AngII, and the protective effect of C. sinensis may be inhibiting the activation of caspase-3 during the AngII-induced apoptosis of NRK-52E.
