Effect of Gingkgo biloba leaf extract induced delayed preconditioning on cytochrome c oxidase expression during myocardial ischemia-reperfusion in rats.
10.3969/j.issn.1672-7347.2012.01.016
- Author:
Ke RAN
1
;
Jingjing WAN
;
Donglin YANG
;
Yanying XIAO
;
Yetian CHANG
Author Information
1. Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Drugs, Chinese Herbal;
pharmacology;
therapeutic use;
Electron Transport Complex IV;
metabolism;
Ginkgo biloba;
chemistry;
Ischemic Postconditioning;
methods;
Ischemic Preconditioning, Myocardial;
methods;
Male;
Myocardial Ischemia;
physiopathology;
Myocardial Reperfusion Injury;
metabolism;
prevention & control;
Phytotherapy;
Plant Leaves;
chemistry;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Central South University(Medical Sciences)
2012;37(1):89-93
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effect of Gingkgo biloba leaf extract (EGb761) induced delayed preconditioning on cytochrome c oxidase (CcO) expression during myocardial ischemia-reperfusion in rats.
METHODS:Four groups (10 in each) of Sprague-Dawley male rats were studied. In the sham group, the rats received no treatment. Rats in the ischemia-reperfusion (IR) group were treated with NS (1.0 mL/kg intravenously) 24 h before ischemia. Rats in the M group were treated with EGb761 (100 mg/kg intravenously) 24 h before the ischemia. In the D group , EGb761-treated rats that received the 5-hydroxydecanoate (5-HD), an inhibitor of mitochondrial KATP channels 15 min before the ischemia. The IR, M, and D groups were subjected to ischemia by 30 min of coronary artery occlusion before 2 h of reperfusion. At the end of the reperfusion, myocardial infarct size was measured. CcO was measured by Western blot. The myocardial ultrastructure was observed under the electron microscope.
RESULTS:The infarct size was significantly smaller in the M group [(23.78 ± 4.82)%] than in the I/R group [(37.87 ± 5.92)%] (P<0.05). The CcO protein expression in the myocardium was significantly higher in the M group than in the I/R group(P<0.05). Microscopic examination showed less myocardial damage in the M group than that in the I/R group. The infarct size, CcO protein expression, and myocardial damage had no significant difference between the D group and the I/R group (P>0.05).
CONCLUSION:EGb761 induced delayed preconditioning attenuates myocardial ischemia-reperfusion injury possibly through up-regulating CcO expression in rats.
