Role of NGX6 in Wnt/β-catenin signaling pathway in colon cancer.
10.3969/j.issn.1672-7347.2011.03.008
- Author:
Fen LIU
1
;
Shourong SHENG
;
Xiaoyan WANG
;
Ya PENG
;
Qin GUO
;
Zhiming XIAO
;
Jie LIU
Author Information
1. Department of Digestion Medicine, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Cell Line, Tumor;
Colonic Neoplasms;
genetics;
metabolism;
pathology;
Cyclin D1;
genetics;
Down-Regulation;
Female;
Gene Expression Regulation, Neoplastic;
Humans;
Male;
Membrane Proteins;
genetics;
metabolism;
Molecular Sequence Data;
Proto-Oncogene Proteins c-myc;
genetics;
RNA, Messenger;
genetics;
metabolism;
Transfection;
Tumor Suppressor Proteins;
genetics;
metabolism;
Wnt Signaling Pathway;
genetics
- From:
Journal of Central South University(Medical Sciences)
2011;36(3):235-242
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the role of nasopharyngeal carinoma associated gene 6 (NGX6) in Wnt/β-catenin signaling pathway and to identify whether there is feedback regulation between NGX6 and Wnt signal pathway once the Wnt signal is blocked.
METHODS:pCMV-myc-NGX6 and pCMV-myc were transfected to colon cancer cell line SW62O and the expression of downstream target molecules of Wnt/β-catenin signaling pathway such as c-myc, cyclinD1, and c-jun were detected by Western blot. The eukaryotic expression vector pcDNA3.1 (+)-LEF1(DN) (without N-terminal β-catenin binding domain) was constructed to inhibit Wnt signaling pathway, and the effect was confirmed by Western blot and TCF-4 luciferase report system. The mRNA expression of NGX6 in SW620 was detected by RT-PCR after pcDNA3.1(+)-LEF1(DN) transient transfection.
RESULTS:The expression of above molecules were obviously down-regulated by NGX6 transient transfection in SW620. The eukaryotic expression vector pcDNA3.1(+)-LEF1(DN) showed an inhibitory effect on Wnt/β-catenin signaling pathway. The mRNA expression of NGX6 in SW620 was up-regulated after pcDNA3.1(+)-LEF1(DN) transient transfection.
CONCLUSION:NGX6 could inhibit the expression of Wnt signaling downstream target genes, which maybe related to the potential feedback regulation between NGX6 and Wnt signal pathway.
