Expression and distribution of transforming growth factor β3 in the mouse tooth germ during development after advanced bell stage.
10.3969/j.issn.1672-7347.2011.03.011
- Author:
Guoying QUE
1
;
Heng LI
;
Lei ZHANG
;
Li ZOU
;
Ying WU
Author Information
1. Department of Stomatology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Animals, Newborn;
Immunohistochemistry;
Mice;
Mice, Inbred BALB C;
Molar;
metabolism;
Tissue Distribution;
Tooth Germ;
growth & development;
metabolism;
Transforming Growth Factor beta3;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2011;36(3):254-257
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the expression and distribution of transforming growth factor β3 (TGF-β3) in the mouse tooth germ after advanced bell stage, and to discuss the role of TGF-β3 during the development of tooth germs.
METHODS:BALB/C's mouse tooth germs at 4, 11, and 18 days postnatal (4dpn,11dpn,and 18dpn) were collected and processed for routine fixation, decalcification, embedding, and slicing. The expression of TGF-β3 was detected by immunohistochemisty.
RESULTS:As to 4dpn tooth germ: Positive expression of TGF-β3 was found in enameloblasts, odontoblasts, ambitus of dental pupilla, with weak positive expression in the intermedial of dental papilla. As to 11dpn tooth germ: Positive expression was seen in enameloblasts, with negative expression in odontoblasts and dental papilla. As to 18dpn tooth, positive expression of TGF-β3 was showed in the vessel wall and its surrounding, with negative expression in other areas.
CONCLUSION:The distribution of TGF-β3 expression showed a time-space characteristic during the mouse tooth germ development after advanced bell stage, which may exert a regulatory effect on tooth development and this effect is gradually getting weak with the development of tooth germs.
