Mechanism of DADS in the bystander effect of HSV-tk/GCV suicide gene therapy system in lens epithelial cells.
10.3969/j.issn.1672-7347.2011.04.009
- Author:
Zhongqing LI
1
;
Qian TAN
;
Zhixiang DING
;
Dan LIU
Author Information
1. Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
metabolism;
Allyl Compounds;
pharmacology;
Animals;
Antiviral Agents;
pharmacology;
Bystander Effect;
drug effects;
Cells, Cultured;
Connexin 43;
metabolism;
Disulfides;
pharmacology;
Epithelial Cells;
metabolism;
Ganciclovir;
pharmacology;
Genes, Transgenic, Suicide;
Lens, Crystalline;
cytology;
drug effects;
metabolism;
Plant Oils;
Rabbits;
Simplexvirus;
enzymology;
Thymidine Kinase;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2011;36(4):329-334
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the mechanism and effect of diallyl disulfide (DADS) on the bystander effect of herpes simplex virus kinase/ganciclovir (HSV-tk/GCV) suicide gene therapy system which was mediated by recombinant adeno-associated virus (rAAV) in lens epithelial cells.
METHODS:Immunohistochemistry method was used to determine the distribution of connexin 43 (Cx43) protein in cultured rabbit lens epithelial cells and rabbit lens epithelial cells transfected by HSV-tk suicide gene. Cx43 protein was measured and analyzed in cultured rabbit lens epithelial cells and rabbit lens epithelial cells transfected by HSV-tk suicide gene that was induced by various DADS. Cell survival was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method.
RESULTS:DADS increased the Cx43 protein expression in cultured rabbit lens epithelial cells and rabbit lens epithelial cells transfected by HSV-tk suicide gene, and especially in 50 μmol/L DADS. After combining with DADS, the bystander effect was significantly improved (P<0.05).
CONCLUSION:DADS can elevate the expression of Cx43 protein and enhance the bystander effect of HSV-tk/GCV suicide gene therapy system.
