Treatment of acute liver injury by intrasplenic transplantation of hepatic stem cells combined with heparin in rats.
10.3969/j.issn.1672-7347.2011.05.007
- Author:
Zenghui HUANG
1
;
Shan ZENG
;
Miao OUYANG
;
Juanjuan DONG
;
Yuewen GONG
;
Hong SHEN
Author Information
1. Medical Research Center, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Carbon Tetrachloride;
Carbon Tetrachloride Poisoning;
Heparin;
therapeutic use;
Hepatocytes;
transplantation;
Liver Failure, Acute;
chemically induced;
therapy;
Male;
Rats;
Rats, Sprague-Dawley;
Stem Cell Transplantation;
methods
- From:
Journal of Central South University(Medical Sciences)
2011;36(5):411-416
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the treatment effects of transplanted hepatic progenitor cells (WB-F344 cells) combined with heparin on the acute liver injury in SD rats.
METHODS:A total of 2*10(7) hepatic stem cells (WB-F344) infected with GFP lentivirus and 8 μL heparin were transplanted through the spleen in SD rats with acute liver injury, which was induced by an intraperitoneal injection of CCl4. The liver and spleen tissues underwent fluorescence examination 1 day after the transplantation. The liver functions were tested, and the liver tissues were histopathologically examined on the 3rd, 7th, 14th, and 28th day of the cell transplantation.
RESULTS:The transfected WB-F344 cells expressed GFP 3 days after the lentivirus infection and were found in the rat liver 1 day after the WB-F344 transplantation. The liver function and histopathological recovery of the liver tissues in the group of WB-F344 transplantation were better than those of the control group (P<0.05).
CONCLUSION:Transplantation of hepatic stem cells combined with heparin can promote the liver recovery in rats with acute liver injury induced by CCl4.
