Regulation of invasion behavior of ERK-mediated trophoblast by focal adhesion kinase.
10.3969/j.issn.1672-7347.2011.06.014
- Author:
Xiqian ZHANG
1
;
Shiling CHEN
;
Fuqi XING
Author Information
1. Reproductive Center, Guangdong Women and Children's Health Hospital, Guangzhou 510010, China.
- Publication Type:Journal Article
- MeSH:
Benzoquinones;
pharmacology;
Cell Division;
physiology;
Cell Movement;
physiology;
Extracellular Signal-Regulated MAP Kinases;
antagonists & inhibitors;
metabolism;
Flavonoids;
pharmacology;
Focal Adhesion Protein-Tyrosine Kinases;
antagonists & inhibitors;
metabolism;
Humans;
Lactams, Macrocyclic;
pharmacology;
Phosphorylation;
Rifabutin;
analogs & derivatives;
Signal Transduction;
physiology;
Trophoblasts;
cytology;
physiology
- From:
Journal of Central South University(Medical Sciences)
2011;36(6):559-564
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the role of focal adhesion kinase (FAK) in extracellular signal-regulated kinase (ERK) signaling pathway mediated invadsion of trophoblasts.
METHODS:We established a human extravillous cytotrophoblasts in vitro invasion model. Different concentrations of herbimycin A(FAK inhibitor)and PD98059 (ERK inhibitor) were given to observe the influence on the growth of trophoblast cells, FAK, ERK phosphorylation, and trophoblast invasion abilities.
RESULTS:The expression of phosphorylated FAK in the extravillous cytotrophoblasts (EVCT) was inhibited by herbimycin A in a concentration-dependent manner and expression of phosphorylated ERK1/2 was also partially reduced. PD98059 had no effect on the expression of phosphorylated FAK. Herbimycin A and PD98059 suppressed the in vitro invasion of EVCT to various degrees.
CONCLUSION:ERK signaling pathway may be the common pathway for many invasive signals,and play a key role in the regulation of trophoblast invasion.
