Definition and function identification of nucleus export signal of BRD7.
10.3969/j.issn.1672-7347.2011.07.009
- Author:
Ming ZHOU
1
;
Chi GUO
;
Xiayu LI
;
Jiajin HE
;
Xiaojie XU
;
Heran WANG
;
Ke TANG
;
Li CAO
;
Xiaoling LI
;
Guiyuan LI
Author Information
1. Cancer Research Institute, Central South University, Changsha 410078, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Base Sequence;
COS Cells;
Cell Nucleus;
metabolism;
Chlorocebus aethiops;
Chromosomal Proteins, Non-Histone;
genetics;
metabolism;
Cytoplasm;
metabolism;
Escherichia coli;
genetics;
metabolism;
Green Fluorescent Proteins;
genetics;
Humans;
Molecular Sequence Data;
Nuclear Export Signals;
Recombinant Proteins;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2011;36(7):634-639
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To localize and define the region of nucleus export signal (NES) on BRD7, and determine the role of this region in nucleus export of the external protein.
METHODS:Based on an in vitro expressed model of green fluorescence protein (GFP), we performed DNA walking analysis to set BRD7 into several sections according to the structural characteristics of BRD7, investigated the effect of different sections of BRD7 on nucleus export of GFP, defined the region of nucleus export signal sequence of BRD7, and further ascertained the content of amino acids in BRD7 and potential localization of BRD7 NES by bioinformatics.
RESULTS:B7C1 fragments ranged from aa219 to aa450 in BRD7 were found to target the external protein GFP into the cytoplasm detected by GFP direct fluorescence, which could be inhibited by NES inhibitor Leptomycin B (LMB). This region was rich in hydrophobic amino acid residues but no typical NES with characteristics of leucine-rich sequence by bioinformatics.
CONCLUSION:The region from aa219 to aa450 is primarily defined as an atypical NES in BRD7.
