Effect of Mcl-1 antisense oligonucleotide on Hela cell biology and sensitivity of chemotherapy.
10.3969/j.issn.1672-7347.2011.07.010
- Author:
Shufang LI
1
;
Jie ZHONG
;
Yongzhong SHI
;
Shasha FAN
Author Information
1. Clinic Medical Research Institute, Hunan People's Hospital, Changsha 410005, China. fanger_li@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Apoptosis;
drug effects;
Drug Resistance, Neoplasm;
drug effects;
HeLa Cells;
Humans;
Myeloid Cell Leukemia Sequence 1 Protein;
Oligonucleotides, Antisense;
genetics;
pharmacology;
Proto-Oncogene Proteins c-bcl-2;
genetics;
Transfection
- From:
Journal of Central South University(Medical Sciences)
2011;36(7):640-645
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of myeloid leukemia-1 (Mcl-1) gene on the proliferation and apoptosis of Hela cells and the sensitivity of cervical cancer chemotherapy by antisense technology.
METHODS:Mcl-1 antisense oligonucleotide(AS-ODN)was transfected into Hela cells with lipofectamine 2000. The expression of Mcl-1 was analyzed by Western blot, the cell viability was detected by MTT assay, and apoptosis was evaluated by flow cytometry.
RESULTS:Mcl-1 AS-ODN arrested the cell cycle at G1/S,greatly inhibited the cell growth and induced apoptosis. The sensitivity of Hela cells on chemotherapy was low. There was obvious increase in the apoptosis rate by chemotherapy drugs and growth inhibition rate after inhibiting the expression of Mc1-1.
CONCLUSION:Mcl-1 AS-ODN can not only inhibit Hela cell proliferation and induce apoptosis, but also increase the sensitivity of chemotherapy. Mcl-1 may be a potential target gene for cervical cancer chemotherapy.
