RUNX3 promoter hypermethylation and prognosis of early surgically resected non-small cell lung cancers.
10.3969/j.issn.1672-7347.2011.07.012
- Author:
Yan TANG
1
;
Fang WU
;
Chunhong HU
Author Information
1. Department of Oncology, Second Xiangya Hospital, Central South University, Changsha 41001, China.
- Publication Type:Journal Article
- MeSH:
Aged;
Carcinoma, Non-Small-Cell Lung;
genetics;
surgery;
Core Binding Factor Alpha 3 Subunit;
genetics;
DNA Methylation;
Female;
Humans;
Lung Neoplasms;
genetics;
surgery;
Male;
Middle Aged;
Prognosis;
Promoter Regions, Genetic;
genetics;
Proportional Hazards Models;
Time Factors
- From:
Journal of Central South University(Medical Sciences)
2011;36(7):650-654
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the relation between the promoter methylation status of RUNX3 gene and clinicopathological parameters, prognosis of non-small cell lung cancer (NSCLC).
METHODS:We collected 80 formalin-fixed paraffin-embedded lung cancer tissue samples from NSCLC patients who received postoperative adjuvant chemotherapy with cisplatin. Genomic DNA was extracted through phenol/chloroform extraction. The methylation status of RUNX3 was determined by nested methylation-specific PCR (nMSP). We investigated the pathological and prognostic characteristics of NSCLC stratified by methylation status.
RESULTS:The RUNX3 promoter methylation was observed in 20 of the 80 NSCLC samples (25.0%). Methylation of RUNX3 was more frequent in adenocarcinomas (36%) than in squamous cell carcinomas (11%) (P=0.020). In multivariate Logistic regression, positive RUNX3 methylation status (P=0.011) was found to be independent disease-free survival factor as was N stage (P<0.001). Kaplan-Meier curves showed patients with RUNX3 methylation had a significantly poorer overall survival than those without methylation (P=0.003; log-rank test). In multivariate Cox proportional hazards regression analysis, RUNX3 methylation (RR:2.345, 95% CI:1.30-4.865, P=0.022) was a significant independent prognostic factor for the overall survival.
CONCLUSION:RUNX3 methylation is a significant independent prognostic factor for disease-free survival and overall survival.
