Effect of interleukin-17 and T helper cell 17 on acute heart allograft rejection in mice.
10.3969/j.issn.1672-7347.2011.07.016
- Author:
Zhongzhou SI
1
;
Jianguo WU
;
Zhijun HE
;
Ting LI
Author Information
1. Department of Organ Transplantation of General Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Graft Rejection;
blood;
diagnosis;
immunology;
Heart Transplantation;
adverse effects;
immunology;
Interleukin-17;
blood;
Male;
Mice;
Mice, Inbred BALB C;
Mice, Inbred C57BL;
Random Allocation;
Th17 Cells;
immunology
- From:
Journal of Central South University(Medical Sciences)
2011;36(7):676-681
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the role of T helper cell 17(Th17) cells and their cytokines IL-17 in heart allograft rejection in mice.
METHODS:The heart transplantation models were randomly divided into 2 groups: a allograft group (n=12) and an isograft group (n=12).On the post-operative day (POD) 3 and 7, serum IL-17 level was tested by enzyme-linked immunosorbent assay, and Th17 cells in heart grafts were measured by flow cytometry. The heart grafts were harvested and saved in 10% formalin, and then embedded in paraffin.
RESULTS:Compared with the iso-graft group, the allograft group had a higher serum level of IL-17 on POD3 and POD7 (P<0.05), and the level of IL-17 was higher on POD7 than that on POD3 (P<0.05). The allograft group had more Th17 cells infiltrating in grafts on POD3 and POD7 (P<0.05) and there were more Th17 cells infiltrating on POD7 than that on POD3 (P<0.05). Histological examination showed that the prolongation of post transplantation time resulted in more rejection pathological changes.
CONCLUSION:Th17 cells may play an important role in the development of heart transplant rejection. IL-17 may serve as a predictive parameter for allograft rejection in the future.
