Effect of Danshensu on fibronectin and collagen-1 secretion induced by high glucose in human peritoneal mesothelial cells.
10.3969/j.issn.1672-7347.2011.01.007
- Author:
Hao ZHANG
1
;
Yi XU
;
Jianwen WANG
;
Ke ZHANG
;
Bin YI
;
Yan LIU
;
Xu CAI
Author Information
1. Department of Nephrology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Cell Line;
Cells, Cultured;
Collagen Type I;
metabolism;
Drugs, Chinese Herbal;
pharmacology;
Epithelial Cells;
metabolism;
Fibronectins;
metabolism;
Glucose;
pharmacology;
Humans;
Lactates;
pharmacology;
Peritoneum;
cytology
- From:
Journal of Central South University(Medical Sciences)
2011;36(1):44-50
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the possible role of Danshensu on fibronectin (FN) and collagen-I (Col-I) secretion induced by high glucose in human peritoneal mesothelial cells (HPMCs).
METHODS:HPMCs were treated with high glucose and Danshensu at different concentrations. The mRNA expression of FN, Col-I, endothelin-1 (ET-1), and heme oxygenase-1 (HO-1) were examined by reverse transcription-polymerase chain reaction (RT-PCR). The protein expression of FN, Col-I HO-1 and ET-1 were analyzed by enzyme-linked immunosorbent assay or immunofluorescence method.
RESULTS:The expression of protein and mRNA of FN and Col-I were attenuated by Danshensu in both dose-dependent and time-dependent manner. The mRNA and protein levels of ET-1 were decreased, and the mRNA and protein levels of HO-1 increased in the Danshensu groups in a dose-dependent manner compared with the high glucose group. The expression of ET-1 and HO-1 showed little difference in a time gradient of danshensu(P>0.05).
CONCLUSION:Danshensu can protect HPMCs through inhibiting the expression of FN and Col-I induced by high glucose, which is related to the suppression of oxidative stress.
