Effect of apolipoprotein A-I mimetic peptides on cholesterol efflux in RAW264.7 cells.
10.3969/j.issn.1672-7347.2011.01.008
- Author:
Qiong XIE
1
;
Shuiping ZHAO
Author Information
1. Department of Cardiology, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
ATP Binding Cassette Transporter 1;
ATP-Binding Cassette Transporters;
metabolism;
Apolipoprotein A-I;
chemistry;
pharmacology;
Biological Transport;
drug effects;
physiology;
Biomimetics;
Cell Line;
Cholesterol;
metabolism;
Cyclic AMP;
metabolism;
Humans;
Macrophages;
cytology;
metabolism;
Peptides;
chemistry;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2011;36(1):51-57
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effect and possible mechanism of an apolipoprotein (apo) A-I mimetic peptide, D-4F, on cholesterol efflux in RAW264.7 macrophages.
METHODS:RAW264.7 macrophages were incubated in the medium containing 8-bromo cAMP (8-Br-cAMP, 0.5 mmol/L) and ox-LDL (50 μg/mL) for 24 h. Then various concentrations of D-4F (0-100 μg/mL) or H89 (20 μmol/L, a protein kinase A inhibitor) were added for the purpose of interference. The intracellular cyclic AMP (cAMP) level was determined by enzyme-linked immunoabsobant assay (ELISA). ATP binding cassette transporter A1 (ABCA1) expression in the macrophages was quantitated by real-time PCR and Western blot.
RESULTS:D-4F significantly increased the cholesterol efflux in both concentration and time-dependent manner accompanied by the increase in the intracellular cAMP level, ABCA1 mRNA and protein expression. The effect of D-4F on cholesterol efflux ABCA1 expression was enhanced by 8-Br-cAMP. Although H89 did not affect the basal cholesterol efflux and ABCA1 expression, it could attenuate the effect of 8-Br cAMP.
CONCLUSION:D-4F affects cholesterol efflux, cAMP level, and ABCA1 expression in macrophages, which is likely involved in the pathway of cAMP/PKA/ABCA1.
