Nerve growth factor increases sodium current via interferon regulatory factor-1 pathway in rat pheochromocytoma cells.
10.3969/j.issn.1672-7347.2011.08.014
- Author:
Yemu ZHU
1
;
Pinhua PAN
;
Hongyi TAN
;
Chengping HU
Author Information
1. Department of Respiratory Medicine, Xiangya Hospital, Central Sounth Univerdity, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Interferon Regulatory Factor-1;
genetics;
metabolism;
Nerve Growth Factor;
pharmacology;
PC12 Cells;
RNA, Messenger;
genetics;
metabolism;
Rats;
Real-Time Polymerase Chain Reaction;
Sodium Channels;
drug effects
- From:
Journal of Central South University(Medical Sciences)
2011;36(8):776-781
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of nerve growth factor(NGF) and interferon regulatory factor-1(IRF-1) on sodium current change of sensory neuron in rat pheochromocytoma cells.
METHODS:Sensory neuron rat pheochromocytoma cells were stimulated by different concentrations of NGF(0-200 ng/mL), the IRF-1 mRNA levels were examined by real-time PCR, and the activation of IRF-1 was examined by Western blot. The sodium current change was recorded by patch clamp.
RESULTS:Low concentration of NGF improved the sodium current, which was concentration dependent. When exposed to high concentration of NGF, the expression of IRF-1 mRNA in PC-12 was improved. Low concentration of NGF resulted in IRF-1 intronuclear transporting, and the expression was not affected. Sodium current did not occur in PC-12 cells when IRF-1 was blocked.
CONCLUSION:NGF can improve the sodium current in PC-12 cells concentration-dependently, and the improvement is regulated by IRF-1.
